K31432

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K31432

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • decreased size (PATO:0000587)
    (Seed::Shape::small)
  • decreased length (PATO:0000574)
    (Seed::Shape::short)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K31432

Determination of introduced cDNA(s)

  • J013122O17 - Rice Full-length cDNAs - Original: J013122O17

    full reading [+] show sequence

    Nucleotide sequence: J013122O17 - full reading 
    GCCCCTCTCT CTCCTTCAAA GCAGGAGAAA AGAAGAAAAA TTGATTGCTC 
    TCTCGTTCCG TTCCGGAGGA GAGAGAGAGA GAGAGAGAGA GGAGAGCGGC 
    GGCGATGGCG TCGTCGTCGT CGTCGGCGTC GAGCAGGTTC TCGCCGGCGC 
    TGCAGGCGTC GGATCTCAAC GACTTCATCG CCCCCTCGCA GGACTGCATC 
    ATCTCCCTCA ACAAGGGCCC CTCCGCCCGC CGCCTTCCGA TCAAACAAAA 
    GGAAATTGCA GTGAGCACAA ATCCTCCAGA GGAAGCAGTC AAGATTTCAC 
    TGAAGGACTG TTTGGCTTGC AGTGGTTGCA TAACATCAGC AGAAACAGTT 
    ATGCTTGAGA AGCAAAGCTT GGGTGACTTC ATTACTCGCA TCAACTCTGA 
    CAAAGCTGTC ATAGTATCGG TCTCTCCACA GTCAAGAGCA TCGCTAGCTG 
    CATTCTTTGG TCTTTCTCAA TCACAGGTTT TCAGAAAACT CACTGCGCTG 
    TTCAAGTCGA TGGGCGTAAA GGCAGTTTAC GACACAAGTT CCAGTCGAGA 
    TCTGTCCCTA ATTGAAGCAT GCAGTGAATT TGTTACTCGT TATCATCAAA 
    ACCAGTTATC TAGTGGCAAA GAAGCTGGAA AAAATCTTCC CATGCTTTCA 
    TCTGCATGCC CAGGTTGGAT ATGCTATGCA GAAAAGACTC TCGGTTCATT 
    TATCTTGCCT TACATCTCAG CTGTGAAGAG CCCCCAACAG GCCATTGGTG 
    CTGCTATCAA GCATCATATG GTTGGAAAAC TTGGTCTGAA GCCACATGAT 
    GTTTATCATG TTACTGTGAT GCCCTGTTAT GATAAAAAAC TAGAAGCTGT 
    TAGGGATGAT TTTGTTTTCT CAGTGGAAGA TAAAGATGTT ACAGAGGTAG 
    ATTCCGTATT AACCACTGGT GAAGTGTTAG ATTTGATACA GTCCAGATCT 
    GTTGATTTCA AGACCCTAGA GGAGTCTCCC ATGGACAGAT TATTAACCAA 
    TGTTGATGAT GACGGTCAAC TATATGGGGT TTCTGGTGGC TCAGGGGGTT 
    ATGCAGAAAC AGTCTTTCGA CATGCGGCAC ATGTTCTCTT CGATAGAAAA 
    ATTGAAGGAT CAGTTGATTT CAGAATCTTA CGAAATTCAG ATTTTCGTGA 
    GGTTACGCTG GAGGTGGAGG GCAAACCTGT TCTTAAGTTT GCACTTTGTT 
    ATGGATTCAG AAATCTGCAG AACATTATTA GGAAGATTAA GATGGGAAAA 
    TGCGAATATC ACTTTATTGA AGTTATGGCA TGTCCTTCAG GCTGCCTGAA 
    TGGGGGAGGT CAAATAAAAC CTGCCAAAGG CCAGTCTGCG AAGGATCTTA 
    TCCAACTACT AGAGGATGTG TACATCCAAG ATGTATCGGT ATCCAACCCC 
    TTCGAGAACC CTATTGCGAA AAGATTATAT GATGAATGGC TTGGGCAACC 
    TGGTTCAGAA AACGCAAAGA AGTATCTACA CACGAAGTAT CACCCAGTGG 
    TGAAAAGCGT GGCATCGCAG TTGCAGAATT GGTGATAACA CAGTGGTGGA 
    AAGCATTTCC CAGTGTGGGC AGTTGCTTGT GCCATCTTGG TGCCCTGCCC 
    ACACTAAGCA AACACTGAAC ACACAGAAAA GAAATACACG TCATCAAAGA 
    TTTTGCATGC GGGCAAATGA ATTTTGTTAG ATTGTAGGTT TTACCAGCTT 
    TTCTTGGTAA TGTTTGTATA CTACCTCCTC ACTGGGAAAC ACAGATGAGA 
    TTGTAACATG TAAAAACGAA GTGAACCAAA TAGTAGTTGA TCTACTCAGC 
    TG

    Gene models with high sequence identity

    1. AT4G16440.1 [+] show detail - ferredoxin hydrogenases
      E-value: 3e-172; Score: 602.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Iron hydrogenase, small subunit-like 2.8e-10
    superfamily Iron hydrogenase 2.1e-127
    HMMPfam Iron hydrogenase, large subunit, C-terminal 7.5e-93
    HMMPfam Iron hydrogenase, small subunit-like 1.8e-10
    Gene3D no description 6.5e-45
    Gene3D Iron hydrogenase, small subunit-like 0.00028
    HMMPanther NARF-RELATED 1.5e-169
    HMMPanther NITRATE, FROMATE, IRON DEHYDROGENASE 1.5e-169

    See the detailed result >

    AT4G16440.1

    Model type
    Protein coding
    Short Description
    ferredoxin hydrogenases
    Curator Summary
    Encodes a [FeFe]-hydrogenase-like protein named Gollum (for Growth in different Oxygen LeveLs inflUences Morphogenesis). Heterologous expression of Gollum in E. coli indicates that it probably contains two [Fe-S] clusters with different magnetic properties. Sequence alignment analysis indicates that these two clusters would be topologically equivalent to the mesial and proximal [Fe-S] centers of [FeFe]-hydrogenases. Knockdown mutants (RNAi) show a dwarf phenotype at the normal atmospheric partial oxygen pressure of 21 kPa. This dwarf phenotype could be rescued by growing the plant under low oxygen pressure (5kPa), suggesting a role for this gene in oxygen sensing.
    Computational Description
    ferredoxin hydrogenases; CONTAINS InterPro DOMAIN/s: Iron hydrogenase, large subunit, C-terminal (InterPro:IPR004108), Iron hydrogenase (InterPro:IPR009016), Iron hydrogenase, small subunit-like (InterPro:IPR003149); Has 2574 Blast hits to 2544 proteins in 612 species: Archae - 1; Bacteria - 1826; Metazoa - 221; Fungi - 188; Plants - 72; Viruses - 0; Other Eukaryotes - 266 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR