K20937

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K20937

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • low saturation (PATO:0000328)
    (Seed::Color::pale)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K20937

Determination of introduced cDNA(s)

  • J023121E06 - Rice Full-length cDNAs - Original: J023121E06

    full reading [+] show sequence

    Nucleotide sequence: J023121E06 - full reading 
    TGGGACCTAC TCTGAAGTAG TACGCGCCGC TACCCTGCCT CTTCTTCCTT 
    CCCACTACCA AACCCGACCA AATATTGTAT TGCTGGCTCG CTGCTTGGGA 
    AGATAAAAGT GCGGCTCTGA AGGGGAATTT CGTCGAACAG GATGAATGCG 
    TTGGCAGCGA CGAGCAGGAA CTTCAAGCAG GCGGCCAAGC TGCTGGGCCT 
    GGACTCCAAG CTGGAGAAGA GCTTGCTCAT CCCGTTCAGG GAGATCAAGG 
    TTGAATGCAC AATTCCGAAA GATGATGGGA CTTTAGCATC TTATGTTGGG 
    TTTAGGGTCC AACACGACAA TGCTAGGGGA CCTATGAAGG GTGGAATCAG 
    ATACCACCAT GAGGTTGATC CTGATGAGGT CAATGCGTTG GCACAACTAA 
    TGACATGGAA GACGGCCGTG GCTAATATTC CATATGGAGG AGCTAAGGTG 
    GAATAGGATG CAGCCCTGGA GATCTCAGCA TCTCCGAGCT AGAGCGACTT 
    ACCCGTGTTT TCACCCAGAA AATCCACGAC TTGATTGGCA TTCATACAGA 
    TGTTCCAGCT CCAGATATGG GAACCAACTC TCAGACAATG GCGTGGATAC 
    TTGACGAATA CTCAAAGTTT CATGGTTATT CACCTGCTGT GGTGACTGGA 
    AAACCTGTTG ACCTTGGAGG ATCACTTGGA AGAGATGCAG CTACTGGAAG 
    GGGAGTTCTG TTTGCTACTG AAGCCTTGCT TGCAGAGCAT GGCAAGGGCA 
    TTGCTGGCCA GCGTTTTGTA ATACAGGGAT TTGGTAATGT TGGATCCTGG 
    GCTGCTCAGC TGATCAGTGA AGCTGGTGGC AAGGTGATTG CCATCAGTGA 
    TGTCACTGGA GCTGTCAAGA ACAGCAATGG GCTTGACATT GCAAAGCTAA 
    TGAAGCACTC ATCAGAGAAC CGCGGGATCA AGGGTTTCGA CGGAGGCGAT 
    GCAATCGACC CACGCTCACT GCTTACTGAA GAATGTGATG TCCTCGTCCC 
    GGCAGCACTT GGTGGAGTTA TAAACAAGGA CAACGCAAAT GAGATCAAAG 
    CAAAGTACAT CATTGAGGCT GCAAACCACC CCACAGACCC TGAGGCAGAT 
    GAGATCCTGT CCAAGAAAGG CGTTCTCATT CTTCCAGACA TCCTCGCGAA 
    CTCCGGTGGT GTCACAGTGA GCTACTTCGA GTGGGTTCAG AACATTCAGG 
    GTTTCATGTG GGACGAGGAG AAGGTGAACA ATGAGCTGAA GACCTACATG 
    ACCCGTGGGT TCAGGGATGT GAAGGAGATG TGCAGGAGCC ACCACTGCGA 
    CCTCCGCATG GGCGCCTTCA CCCTCGGTGT CAACCGTGTC GCTCGCGCCA 
    CCGTTCTCAG AGGATGGGAA GCATGATCTC GTCAGTCATC TGATCATCTC 
    CCTGTTGTAC TTCATGCACT GAATTTCTTT CGGTTTTTCG CTTTGGTTAA 
    TCCGTCAACC GGGATTGGAG AAGATAGATT CAATAACCAA CCCCACGATG 
    AGTCGATGAG TGTGCTGCCT TTCTCAGAGA TTAGTTGGGG GAAGAAACAG 
    TAGTAATCTG CTGGGTAGTG ATGAATTGCC TGAATTTATC ATCTTGCAGA 
    ATGGCACTGC AGGATTTCTT GTACTCTGTG GCTGAAATTC TTGCTGGTTT 
    GCAAGCAGTA ATCTGAATTT GGTTTCTTGG CT

    Gene models with high sequence identity

    1. AT5G18170.1 [+] show detail - glutamate dehydrogenase 1
      E-value: 0; Score: 196.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Glutamate/phenylalanine/leucine/valine dehydrogenase, dimerisation domain 2.3e-23
    HMMPfam Glutamate/phenylalanine/leucine/valine dehydrogenase, C-terminal 1e-74
    HMMPfam Glutamate/phenylalanine/leucine/valine dehydrogenase, dimerisation domain 3.1e-14
    FPrintScan Glutamate/phenylalanine/leucine/valine dehydrogenase 6.2e-27
    FPrintScan Glutamate/phenylalanine/leucine/valine dehydrogenase 6.2e-27
    FPrintScan Glutamate/phenylalanine/leucine/valine dehydrogenase 6.2e-27
    superfamily Aminoacid dehydrogenase-like, N-terminal doma 2.3e-30
    superfamily NAD(P)-binding domain 3e-75
    superfamily Aminoacid dehydrogenase-like, N-terminal doma 4.7e-24
    Gene3D no description 7e-23
    Gene3D no description 3.4e-18
    Gene3D NAD(P)-binding domain 7.9e-82
    HMMSmart Glutamate/phenylalanine/leucine/valine dehydrogenase, C-terminal 1.8e-93
    HMMPanther Glutamate/phenylalanine/leucine/valine dehydrogenase 3e-152
    HMMPanther GLUTAMATE DEHYDROGENASE 3e-152
    HMMPanther Glutamate/phenylalanine/leucine/valine dehydrogenase 2.8e-47
    HMMPanther GLUTAMATE DEHYDROGENASE 2.8e-47

    See the detailed result >

    AT5G18170.1

    Model type
    Protein coding
    Short Description
    glutamate dehydrogenase 1
    Curator Summary
    Encodes the 43 kDa alpha-subunit of the glutamate dehydrogenase with a putative mitochondrial transit polypeptide and NAD(H)- and alpha-ketoglutarate-binding domains. Mitochondrial localization confirmed by subcellular fractionation. Combines in several ratios with GDH2 protein (GDH-beta) to form seven isoenzymes. Catalyzes the cleavage of glycine residues. May be involved in ammonia assimilation under conditions of inorganic nitrogen excess. The enzyme is almost exclusively found in the mitochondria of stem and leaf companion cells.
    Computational Description
    glutamate dehydrogenase 1 (GDH1); FUNCTIONS IN: in 6 functions; INVOLVED IN: response to cadmium ion, response to salt stress, nitrogen compound metabolic process, response to absence of light; LOCATED IN: mitochondrion; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glutamate/phenylalanine/leucine/valine dehydrogenase (InterPro:IPR006095), Glutamate/phenylalanine/leucine/valine dehydrogenase, C-terminal (InterPro:IPR006096), NAD(P)-binding domain (InterPro:IPR016040), Glutamate dehydrogenase (InterPro:IPR014362), Glutamate/phenylalanine/leucine/valine dehydrogenase, dimerisation domain (InterPro:IPR006097); BEST Arabidopsis thaliana protein match is: glutamate dehydrogenase 3 (TAIR:AT3G03910.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR