K06334

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: K06334

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: K06334

Determination of introduced cDNA(s)

  • J013146N15 - Rice Full-length cDNAs - Original: J013146N15

    full reading [+] show sequence

    Nucleotide sequence: J013146N15 - full reading 
    GCGCGGCGTG GGTGCGGGGG TTAAACAAGG GAAGGGGTTT TTGGCGTCAC 
    TCGCTTCTCT CTCTCCCCTA CGCGCCGCCG GCGAGATGCG GAGCCTCGCC 
    GTCGCGAGCC CCCTCCCGCC CGCCGCCGCG GTTCGCCGCC GGCCACGCGC 
    ATCCGCCTCC GGAAGGGAAG TGATTTCACA ATGTTGGAAG TGTGAGATAA 
    ATCAGGACCA ACCTTTGGGG AACTCCCTAA GGATAGGTCA CTCACAAGGT 
    AGTTTGCAAA GACATGGTTC CAGGAATTTG CTCGCAGCTG CTGCGGCTAT 
    TTCAATTGAA CAAGCTGAAG TCAGTACTTA TCTTCCAAAA GGTGACATGT 
    GGTCTGTGCA CAAGTTTGGT GGCACCTGTA TGGGAACACC CCAGAGAATT 
    CAGAATGTTG CTGATATTGT TCTTGGTGAC TCTTCTGAGA GGAAGCTGAT 
    CATTGTTTCT GCAATGTCCA AAGTCACTGA CATGATGTTC AACCTTGTGC 
    ATAAAGCTCA GTCAAGGGAT AATTCTTATG TGACTGCACT GGATGAAGTT 
    TTTAATAAGC ATATGGCTGC AGCAAAGGAA CTTCTTGATG GAGAAGACCT 
    AGCAAGATTC TTGGCTCAGT TGCATTCTGA CATCAGTAAC CTGAGAGCGA 
    TGCTTCGTGC TATTTTTATA GCTGGACATG CAACAGAATC GTTCTCTGAT 
    TTCGTTGTTG GGCATGGAGA GTTGTGGTCA GCTCAAATGT TGTCGTATGC 
    TATAAAAAAG TCTGGGGTGC CTTGCAGTTG GATGGATACA AGAGAAGTTC 
    TAGTAGTGAA ACCTAGTGGT TCTAATCAGG TCGATCCTGA CTATTTGGAA 
    TCTGAGAAGC GACTCCAGAA ATGGTTTTCA CGACAACCAG CAGAGATAAT 
    AATCGCTACT GGGTTTATTG CCAGTACAGC CGAAAACATT CCTACTACGC 
    TTAAAAGGGA TGGAAGCGAC TTCTCAGCAT CAATAATTGG ATCCCTTGTT 
    AGGGCATGTC AGGTCACTAT CTGGACTGAT GTTGATGGGG TATTTAGTGC 
    AGATCCTAGA AAAGTTAGTG AGGCTGTGAT CTTAAGCACA TTATCTTATC 
    AGGAGGCCTG GGAAATGTCA TATTTTGGTG CAAATGTTTT GCATCCCCGC 
    ACCATTATAC CTGTGATGAA AGACAATATT CCTATTGTCA TTAGGAATAT 
    GTTCAATCTA TCTGCCCCTG GAACAACAAT TTGCAAGCAG CCTGCTAATG 
    AAAATGCTGA TTTAGATGCT TGTGTCAAAT CATTTGCTAC TATAGACAAA 
    TTGGCCCTTG TTAATGTGGA AGGAACTGGA ATGGCTGGTG TTCCAGGTAC 
    AGCAAGTGCC ATATTTAGTG CTGCCAAGGA TGTTGGAGCT AATGTTATCA 
    TGATATCTCA GGCTAGCAGC GAACACTCGG TATGCTTTGC TGTTCCAGAA 
    AAGGAAGTTG CAGCAGTCTC GACAGCCTTG CATGTTAGGT TTCGTGAGGC 
    ATTAGCAGCT GGAAGGCTAT CTAAGGTCGA AGTCATTCGT GGGTGCAGCA 
    TTCTTGCTGC TGTTGGCTTG AGGATGGCCA GTACTCCTGG AGTCAGTGCA 
    ATCCTTTTTG ATGCATTAGC AAAGGCAAAT ATTAATGTAC GAGCAATAGC 
    CCAAGGTTGC AGTGAATACA ATATTACTGT TGTGTTGAAG CAAGAAGATT 
    GTGTGAGGGC TTTGAGAGCT GTTCATTCAA GGTTCTTCCT CTCCAAAACC 
    ACTCTTGCCG TTGGGATCAT TGGACCAGGT TTAATTGGTG GAACACTTCT 
    TGACCAGCTG AAAGATCAGG CTGCTGTTCT CAAGGAAAAT ATGAATATTG 
    ATCTACGTGT TATTGGAATA TCTGGCTCAA GAACGATGCA TTTGAGTGAC 
    ATAGGGGTAG ACTTAAACCA GTGGAAAGAG CTTCTACGAA AAGAAGCAGA 
    ACCAGCAGAT CTTGATAGTT TTGTTCGCCA TTTGTCTGAA AATCATGTAT 
    TTCCAAACAA AGTTTTGGTG GACTGCACAG CAGATACATA CGTAGCATGC 
    CACTATTATG ATTGGTTGAA GAAGGGTATC CATGTCATCA CGCCAAATAA 
    GAAAGCAAAT TCTGGTCCGC TTGATCGGTA TCTGAAATTA AGGACTCTGC 
    AGCGTGCATC GTATACTCAT TACTTTTATG AAGCAACTGT TGGTGCTGGC 
    CTTCCAATCA TTAGCACCCT ACGAGGTCTT CTAGAGACAG GTGACAAGAT 
    ATTGCGTATT GAGGGCATTT TCAGTGGGAC TTTGAGTTAT ATTTTCAACA 
    ATTTTGAAGG TACACGAACT TTTAGTAATG TTGTTGCTGA AGCAAAAGAA 
    GCTGGATATA CAGAGCCAGA TCCAAGAGAT GATCTATCAG GAACTGATGT 
    TGCCAGAAAG GTTATTATCC TTGCAAGGGA GTCTGGTCTG AGGCTCGAGC 
    TTTCCGATAT TCCCGTAAAG AGCCTTGTGC CAGAGGCACT GAGGTCATGT 
    TCGTCAGCAG ATGAATTCAT GCAGAAGCTA CCGTCCTTTG ATCAGGACTG 
    GGACAGGCAA CGCGATGAAG CTGAAGCTGC AGGAGAAGTG CTGCGGTATG 
    TGGGCGTGGT GGACGTGGCG AACAGGAAGG GCCGGGTAGA GCTGCAGAGG 
    TACAAGAGAG ACCACCCCTT CGCGCAGCTG TCCGGCTCCG ACAACATCAT 
    CGCCTTCACC ACGTCGAGGT ACAAGGAGCA GCCGCTGATC GTGCGAGGCC 
    CCGGCGCCGG CGCGGAGGTC ACCGCCGGTG GCGTCTTCTG CGACATCCTG 
    CGGCTGGCGT CCTACCTGGG AGCACCCTCC TAGGCTTTAA CCTTGAGCGT 
    CCTGCCTTCC TCGACAAAAA TTGTCTGGAG GAAGATGAGG CGTATTGATT 
    GTGCCCGACT GGTTATTACA TCATATATGG GACTGGTAGT ATGCAGTATG 
    TATGTTGTTT CTGAATAAGT GCGAGGAAAT AAGGTCTTAG AGGTGCTAGA 
    TGGTCCTTGG ATCAGGTGTT GTTTTTGCCA CACAATCCAG AGCCGCTTGC 
    TATCGAACTG CCACATTGTA TTGGTGATTT GGAGTGATCT AATAATAATG 
    GCTTAAATTT CTTTTTGCTG

    Gene models with high sequence identity

    1. AT4G19710.2 [+] show detail - aspartate kinase-homoserine dehydrogenase ii
      E-value: 0; Score: 999.99

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Homoserine dehydrogenase, catalytic 3.2e-57
    HMMPfam Aspartate/glutamate/uridylate kinase 8e-45
    HMMPfam Aspartate/homoserine dehydrogenase, NAD-binding 6.2e-29
    HMMPfam Amino acid-binding ACT 1.3e-08
    HMMPfam Amino acid-binding ACT 1.9e-08
    Gene3D Aspartate/glutamate/uridylate kinase 1.3e-11
    Gene3D NAD(P)-binding domain 1.8e-58
    superfamily Glyceraldehyde-3-phosphate dehydrogenase-like 3.9e-56
    superfamily NAD(P)-binding domain 6.2e-41
    superfamily Aspartate/glutamate/uridylate kinase 2.4e-24
    HMMPanther ASPARTATE KINASE 0
    HMMPanther ASPARTATE KINASE 0
    PatternScan Aspartate kinase, conserved site 0
    PatternScan Homoserine dehydrogenase, conserved site 0
    HMMTigr Aspartate kinase domain 2.4e-112

    See the detailed result >

    AT4G19710.2

    Model type
    Protein coding
    Short Description
    aspartate kinase-homoserine dehydrogenase ii
    Curator Summary
    Encodes a bifunctional aspartate kinase/homoserine dehydrogenase. These two activities catalyze the first and the third steps toward the synthesis of the essential amino acids threonine, isoleucine and methionine.
    Computational Description
    aspartate kinase-homoserine dehydrogenase ii (AK-HSDH II); FUNCTIONS IN: homoserine dehydrogenase activity, aspartate kinase activity; INVOLVED IN: aspartate family amino acid biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aspartate/glutamate/uridylate kinase (InterPro:IPR001048), Homoserine dehydrogenase, catalytic (InterPro:IPR001342), Amino acid-binding ACT (InterPro:IPR002912), Aspartate/homoserine dehydrogenase, NAD-binding (InterPro:IPR005106), Aspartate kinase, conserved site (InterPro:IPR018042), NAD(P)-binding domain (InterPro:IPR016040), Aspartate kinase domain (InterPro:IPR001341), Bifunctional aspartokinase/homoserine dehydrogenase I (InterPro:IPR011147), Homoserine dehydrogenase, conserved site (InterPro:IPR019811); BEST Arabidopsis thaliana protein match is: aspartate kinase-homoserine dehydrogenase i (TAIR:AT1G31230.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR