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GT22218

CSHL Arabidopsis genetrap mutant lines

Original: GT22218

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT22218

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 3; Position: 3521080 [Map]; Strand: c
      • AT3G11240.1 - [+] show detail - arginine-tRNA protein transferase 2
        Distance: 0; Region: exon1 (CDS)
      • AT3G11250.1 - [+] show detail - Ribosomal protein L10 family protein
        Distance: -277; Region: promoter
    Flanking Genome Sequence: GT22218 - 5-prime edge
    TTTTCTCTCT GTCGACGAGA GCTCACCATG ATTCTCATCG TTGTGGATTC 
    TCTCCGATCA TGTCGTCGAA GAAAGCGAAG ACGAAGAACG AGGCGAGTAG
    CAGCCGTGGT GGCATCGGCG GAGAGAGTAT AGTCGCTGAT TGTGGACGAA
    ATAAATCAAC TTGCGGTTAC TGTAAATCTT CTACCCGCTT TAGTATTTCT
    CACGGTTCGT TGCATTGCCT TTGAAATATC GTTTTATTAG TTCGTGAATC
    TTCTGTATAG TTTGTCGTTG AATTGATCTG ACTATCGTAT AAGTCAAATA
    AAAAGATTTT CCGATTTGGG AATGTGAATG ATTTCCATTG CAGGTTTATG
    GACAGAGAGA CTCACCGTTA ATGACTATCA AGGTTTGTTA CATCAATCTC
    ATGGTCTTAT TAATATGTTT GATTATGTGC AAACACAAAG TTTGAATTTT
    TGCTTCTTTG ATTTGAAGTT CTTCATTCAA CATTTTGATT CTCCTTTCTG
    AGCTTAGTCA TCATTTTTGA TGTATCTTTT TTTTTTTTTT TTTTTAAAAA
    CCCTTTTTTT CCCGGGAAAG GAAAAATTTT GGCTTCCCTT TTTAAAAACG
    GGAGAGGGAA AAATTTTTGT CCCTTTTTT

AT3G11240.1

Model type
Protein coding
Short Description
arginine-tRNA protein transferase 2
Curator Summary
Encodes an arginyl-tRNA:protein arginyltransferase (ATE2), a component of the N-end rule pathway that targets protein degradation through the identity of the amino-terminal residue of specific protein substrates. Arabidopsis contains two ATE genes: At5g05700/ATE1, At3g11240/ATE2. Another component of the N-end rule pathway is At5g02310/PROTEOLYSIS6 (PRT6). PRT6 and ATE were shown to regulate seed after-ripening, seedling sugar sensitivity, seedling lipid breakdown, and abscisic acid (ABA) sensitivity of germination.
Computational Description
arginine-tRNA protein transferase 2 (ATE2); FUNCTIONS IN: arginyltransferase activity; INVOLVED IN: regulation of seed germination, regulation of lipid catabolic process, protein arginylation, response to abscisic acid stimulus; LOCATED IN: cellular_component unknown; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: Arginine-tRNA-protein transferase, N-terminal (InterPro:IPR007471), Arginine-tRNA-protein transferase 1, eukaryotic (InterPro:IPR017137), Arginine-tRNA-protein transferase, C-terminal (InterPro:IPR007472); BEST Arabidopsis thaliana protein match is: arginine-tRNA protein transferase 1 (TAIR:AT5G05700.1); Has 2270 Blast hits to 2031 proteins in 783 species: Archae - 0; Bacteria - 1438; Metazoa - 213; Fungi - 241; Plants - 48; Viruses - 0; Other Eukaryotes - 330 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G11250.1

Model type
Protein coding
Short Description
Ribosomal protein L10 family protein
Computational Description
Ribosomal protein L10 family protein; FUNCTIONS IN: structural constituent of ribosome; INVOLVED IN: translational elongation, response to salt stress, translation; LOCATED IN: cytosolic ribosome, ribosome, membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Ribosomal protein 60S (InterPro:IPR001813), Ribosomal protein L10 (InterPro:IPR001790); BEST Arabidopsis thaliana protein match is: Ribosomal protein L10 family protein (TAIR:AT3G09200.1); Has 1983 Blast hits to 1980 proteins in 500 species: Archae - 322; Bacteria - 1; Metazoa - 680; Fungi - 420; Plants - 226; Viruses - 0; Other Eukaryotes - 334 (source: NCBI BLink).
Link
InterPro Scan - TAIR