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GT21925

CSHL Arabidopsis genetrap mutant lines

Original: GT21925

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT21925

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 24170192 [Map]; Strand: w
      • AT1G65060.1 - [+] show detail - 4-coumarate:CoA ligase 3
        Distance: 0; Region: intron2 (CDS)
      • AT1G65060.2 - [+] show detail - 4-coumarate:CoA ligase 3
        Distance: 0; Region: intron2 (CDS)
    Flanking Genome Sequence: GT21925 - 5-prime edge 
    TTTTTTTTAT TTAGCAAAAT TATTCAAATC TTTAATTTAT TCATTTTCAC 
    CAAAAAATTA TTATAGAATG AAGACTTCAG TAGTGAAAAT AATAAAATGG 
    AGTTAAAATG CTTGTTAGAT TTTTAAAATG GTTCCCGCTA AATTAGTAGT 
    GTAAATTCAA ATAGTAAGGG TCTAAAAACA GAGTAAATAA TAAGCAGAGA 
    TAGAGATAAG ACCTGGCCAA GGATGGCTTG AGGGAGACGG CGACGAAGAC 
    TATCTTGAAG TTCTTTACCT AAAGGAGCTG CACCGGAAAG AACGAATCTA 
    ACGGAGGAGA GATCATAAGA ATTAACCGTC GGGTTTTTAG CTAGAGCGAT 
    CACTAGCGGC GGTACAAGCG CCGCTATTGT CACTCTATGT CTTTGAATAA 
    GATCCAATAA CGCACCGATC TCAAACTTAT GCATTAAAAG AACCGTGGCG 
    CCTATTAAAG GAGTGTTTTA ATAGTTAGGT AACAAAACAT TAAAGATTAT 
    GAATTGAGGA ATACGACGTC GTTTAGTTAC CGGATCGGAG TGAATTGAGG 
    AGGACGCTAT TGAGAGAGTA GATATGGAAA AGTGATAAAA CGCAGAGTAT 
    GACGTCGTTT GATTTGAGGT AAAGATTTGG GTTATCTCCA TCTACTTGTT 
    GTG

AT1G65060.1

Model type
Protein coding
Short Description
4-coumarate:CoA ligase 3
Curator Summary
encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. mRNA levels are not induced in response to wounding or to fungal infection by P. parasitica. mRNA is expressed in flowers, to a lesser degree in mature leaves and siliques and marginally in seedling roots and bolting stems of mature plants. The catalytic efficiency was in the following (descending) order: p-coumaric acid, caffeic acid, ferulic acid, cinnamic acid and 5-OH-ferulic acid. At4CL3 was unable to use sinapic acid as substrate.
Computational Description
4-coumarate:CoA ligase 3 (4CL3); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: 4-coumarate:CoA ligase 2 (TAIR:AT3G21240.1); Has 85516 Blast hits to 77968 proteins in 3765 species: Archae - 1155; Bacteria - 54010; Metazoa - 3516; Fungi - 4668; Plants - 2834; Viruses - 1; Other Eukaryotes - 19332 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G65060.2

Model type
Protein coding
Short Description
4-coumarate:CoA ligase 3
Curator Summary
encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. mRNA levels are not induced in response to wounding or to fungal infection by P. parasitica. mRNA is expressed in flowers, to a lesser degree in mature leaves and siliques and marginally in seedling roots and bolting stems of mature plants. The catalytic efficiency was in the following (descending) order: p-coumaric acid, caffeic acid, ferulic acid, cinnamic acid and 5-OH-ferulic acid. At4CL3 was unable to use sinapic acid as substrate.
Computational Description
4-coumarate:CoA ligase 3 (4CL3); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: 4-coumarate:CoA ligase 2 (TAIR:AT3G21240.1); Has 78268 Blast hits to 71395 proteins in 3651 species: Archae - 1111; Bacteria - 51774; Metazoa - 3299; Fungi - 4373; Plants - 2538; Viruses - 1; Other Eukaryotes - 15172 (source: NCBI BLink).
Link
InterPro Scan - TAIR