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GT19173

CSHL Arabidopsis genetrap mutant lines

Original: GT19173

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT19173

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 3; Position: 17441240 [Map]; Strand: w
      • AT3G47340.1 - [+] show detail - glutamine-dependent asparagine synthase 1
        Distance: 0; Region: exon1 (5UTR)
      • AT3G47340.2 - [+] show detail - glutamine-dependent asparagine synthase 1
        Distance: 0; Region: exon1 (5UTR)
      • AT3G47340.3 - [+] show detail - glutamine-dependent asparagine synthase 1
        Distance: 0; Region: exon1 (5UTR)
      • AT3G47341.1 - [+] show detail - unknown protein; LOCATED IN: endomembrane system; Has 30201 Blast hits to 173...
        Distance: -181; Region: promoter
    Flanking Genome Sequence: GT19173 - 3-prime edge
    TTTACCGACC GTTACCGACC GTTTTCATCC CTAGGCAGAG TAGAGCCAAC 
    ACTATTAAAG CCTCGATTAT TTCAATCAGA TCAACTATAA TACAATGTTA
    CATAATTTCC ATATACCAAG TTACCAACAC GGAAACACCA CCTATATAAC
    AGCTGCAGCG AGTAGCACAG CACTACCAAC AGTTTCGGAA AATTCTGTTT
    TTCTGTTCTC ATTCTTCGTG ATCTTTTTCA CTTTCTTCAA AAAAAAAACA
    TGGGGGGAAT ACTTGCCGGG TTAGGATGTT CCAATGATTC TCAGGCCAGG
    AGAGTTCGGG TTCTTGAGCT TTCTCGCAGG CAATACATTT TTCCTCTGTT
    TTTTTTTGGG TTTTCAAAAA AAAAAAATTT TTTGGTTTGT CACGTTAAAC
    CCTTTCTAAT TTGGGCAAAA TTCCGGGGGG ATTATTTTGA GGTTAAACTT
    TTTAAAATAC AAATTTTTTT GTTTCAAAAA TTTGTTAGGG AAAATTGATG
    TCTTCTTTCT CTTTTTACAC AACCCCGATA TACATATTTT GTTTTTGAAT
    ATTTGTCAAT TTTTTCTTTT TCTTGGCCCT TTTTTACTGA ACGGGACTTG
    AACGGTGAGG CTTGATCAAA ATAACCCTCC CCTTTGGGCT TATTTAATGG
    GGATATCTTT TAAAA

AT3G47340.1

Model type
Protein coding
Short Description
glutamine-dependent asparagine synthase 1
Curator Summary
encodes a glutamine-dependent asparagine synthetase, the predicted ASN1 peptide contains a purF-type glutamine-binding domain, and is expressed predominantly in shoot tissues, where light has a negative effect on its mRNA accumulation. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
glutamine-dependent asparagine synthase 1 (ASN1); CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Asparagine synthase (InterPro:IPR001962), Asparagine synthase, glutamine-hydrolyzing (InterPro:IPR006426), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: asparagine synthetase 2 (TAIR:AT5G65010.1); Has 10466 Blast hits to 10069 proteins in 1927 species: Archae - 313; Bacteria - 6097; Metazoa - 215; Fungi - 282; Plants - 342; Viruses - 7; Other Eukaryotes - 3210 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G47340.2

Model type
Protein coding
Short Description
glutamine-dependent asparagine synthase 1
Curator Summary
encodes a glutamine-dependent asparagine synthetase, the predicted ASN1 peptide contains a purF-type glutamine-binding domain, and is expressed predominantly in shoot tissues, where light has a negative effect on its mRNA accumulation. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
glutamine-dependent asparagine synthase 1 (ASN1); CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Asparagine synthase (InterPro:IPR001962), Asparagine synthase, glutamine-hydrolyzing (InterPro:IPR006426), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: asparagine synthetase 2 (TAIR:AT5G65010.2); Has 10371 Blast hits to 9977 proteins in 1906 species: Archae - 313; Bacteria - 6018; Metazoa - 215; Fungi - 282; Plants - 337; Viruses - 5; Other Eukaryotes - 3201 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G47340.3

Model type
Protein coding
Short Description
glutamine-dependent asparagine synthase 1
Curator Summary
encodes a glutamine-dependent asparagine synthetase, the predicted ASN1 peptide contains a purF-type glutamine-binding domain, and is expressed predominantly in shoot tissues, where light has a negative effect on its mRNA accumulation. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
glutamine-dependent asparagine synthase 1 (ASN1); CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Asparagine synthase (InterPro:IPR001962), Asparagine synthase, glutamine-hydrolyzing (InterPro:IPR006426), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: asparagine synthetase 3 (TAIR:AT5G10240.1); Has 9911 Blast hits to 9877 proteins in 1912 species: Archae - 315; Bacteria - 5703; Metazoa - 210; Fungi - 275; Plants - 333; Viruses - 5; Other Eukaryotes - 3070 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G47341.1

Model type
Protein coding
Computational Description
unknown protein; LOCATED IN: endomembrane system; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR