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GT19170

CSHL Arabidopsis genetrap mutant lines

Original: GT19170

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT19170

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 3; Position: 17441237 [Map]; Strand: w
      • AT3G47340.1 - [+] show detail - glutamine-dependent asparagine synthase 1
        Distance: 0; Region: exon1 (5UTR)
      • AT3G47340.2 - [+] show detail - glutamine-dependent asparagine synthase 1
        Distance: 0; Region: exon1 (5UTR)
      • AT3G47340.3 - [+] show detail - glutamine-dependent asparagine synthase 1
        Distance: 0; Region: exon1 (5UTR)
      • AT3G47341.1 - [+] show detail - unknown protein; LOCATED IN: endomembrane system; Has 30201 Blast hits to 173...
        Distance: -184; Region: promoter
    Flanking Genome Sequence: GT19170 - 3-prime edge
    TTACCGACCG TTACCGACCG TTTTCATCCC TAGTGGTAGA ACACCGAGAC 
    ATCATATACA CGAATATCGA TAGTACACTT CTACGTGCAA TTTTCTCCTT
    TCTCTTCCTG GACATCTGTC TGTTTATTAC ATTTTCTTGT AATCTCTTTT
    TGGGGTTTTA CAATATCTAT CCCCTAAAGT TTCGGAAAAT TCTGTTTTTC
    TGTTCTCATT CTTCGTGATC TTTTTCACTT TCTTCAAAAA AAAAACATGT
    GTGGAATACT TGCCGTGTTA GGATGTTCCA ATGATTCTAA GGCCAAGAGA
    TTTCGTGTTC TTGAGCTTTC TCCCAGGCAA AACATTTTTC CTCTGTTTTT
    TTTTTGGTTT TAAAAAAAAA AAAATTTTTT GTTTTGCCCC TTTAAAACCT
    TTCTAATTTG GGCAAAATTA CTGGATGATA ATTTTAATTT AAAACTTTTA
    AAAAAACTAA TTTTTTTGTT TCAAAATTAT TTAAAGAAAA ATTAATGTCT
    TCTAATTATT TTTACACAAC CCCAATTTAC TTAATTTAAT TTTGAATTAT
    TGCCAAAATT TTCTTTTTCT TGAACCTTTT TTACTAAACT TAACTTAAAC
    AGTGATGTTT AATCAAAAAA AACTCCCCCT TTTTCCAAAT TTAATGTGAA
    TATCTTTAAA AAAATCTAAA TCTTTTTTTT TTGTTGAAAT ATCCCCAAAA
    AAGGTTTTAA TTTTATATAT TTGTATTTTT TTTCAAATTT TTGTTT

AT3G47340.1

Model type
Protein coding
Short Description
glutamine-dependent asparagine synthase 1
Curator Summary
encodes a glutamine-dependent asparagine synthetase, the predicted ASN1 peptide contains a purF-type glutamine-binding domain, and is expressed predominantly in shoot tissues, where light has a negative effect on its mRNA accumulation. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
glutamine-dependent asparagine synthase 1 (ASN1); CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Asparagine synthase (InterPro:IPR001962), Asparagine synthase, glutamine-hydrolyzing (InterPro:IPR006426), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: asparagine synthetase 2 (TAIR:AT5G65010.1); Has 10466 Blast hits to 10069 proteins in 1927 species: Archae - 313; Bacteria - 6097; Metazoa - 215; Fungi - 282; Plants - 342; Viruses - 7; Other Eukaryotes - 3210 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G47340.2

Model type
Protein coding
Short Description
glutamine-dependent asparagine synthase 1
Curator Summary
encodes a glutamine-dependent asparagine synthetase, the predicted ASN1 peptide contains a purF-type glutamine-binding domain, and is expressed predominantly in shoot tissues, where light has a negative effect on its mRNA accumulation. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
glutamine-dependent asparagine synthase 1 (ASN1); CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Asparagine synthase (InterPro:IPR001962), Asparagine synthase, glutamine-hydrolyzing (InterPro:IPR006426), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: asparagine synthetase 2 (TAIR:AT5G65010.2); Has 10371 Blast hits to 9977 proteins in 1906 species: Archae - 313; Bacteria - 6018; Metazoa - 215; Fungi - 282; Plants - 337; Viruses - 5; Other Eukaryotes - 3201 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G47340.3

Model type
Protein coding
Short Description
glutamine-dependent asparagine synthase 1
Curator Summary
encodes a glutamine-dependent asparagine synthetase, the predicted ASN1 peptide contains a purF-type glutamine-binding domain, and is expressed predominantly in shoot tissues, where light has a negative effect on its mRNA accumulation. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
glutamine-dependent asparagine synthase 1 (ASN1); CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Asparagine synthase (InterPro:IPR001962), Asparagine synthase, glutamine-hydrolyzing (InterPro:IPR006426), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: asparagine synthetase 3 (TAIR:AT5G10240.1); Has 9911 Blast hits to 9877 proteins in 1912 species: Archae - 315; Bacteria - 5703; Metazoa - 210; Fungi - 275; Plants - 333; Viruses - 5; Other Eukaryotes - 3070 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G47341.1

Model type
Protein coding
Computational Description
unknown protein; LOCATED IN: endomembrane system; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR