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GT19169

CSHL Arabidopsis genetrap mutant lines

Original: GT19169

Phenotypes

Morphological phenotypes

Original description

  • embryo lethal (unconfirmed)
Anatomical entity
/structure development stage
Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)

Original: GT19169

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 3; Position: 17441238 [Map]; Strand: w
      • AT3G47340.1 - [+] show detail - glutamine-dependent asparagine synthase 1
        Distance: 0; Region: exon1 (5UTR)
      • AT3G47340.2 - [+] show detail - glutamine-dependent asparagine synthase 1
        Distance: 0; Region: exon1 (5UTR)
      • AT3G47340.3 - [+] show detail - glutamine-dependent asparagine synthase 1
        Distance: 0; Region: exon1 (5UTR)
      • AT3G47341.1 - [+] show detail - unknown protein; LOCATED IN: endomembrane system; Has 30201 Blast hits to 173...
        Distance: -183; Region: promoter
    Flanking Genome Sequence: GT19169 - 3-prime edge
    TTTACCGACC GTTACCGACC GTTTTCATCC CTAGTCGTAG AAAACCAAGA 
    CATCAAAAAC ACGAATATCG ATAGTACACT TCTACGTGCA ATTTTCTCCT
    TTCTCTTCCT GGACATCTGT CTGTTTATTA CATTTTCTTG TAATCTCTTT
    TTGGGGTTTT ACAATATCTA TCCCCTAAAG TTTCGGAAAA TTCTGTTTTT
    CTGTTCTCAT TCTTCGTGAT CTTTTTCACT TTCTTCAAAA AAAAAACATG
    TGTGGAATAC TTGCCGTGTT AGGATGTTCC AATGATTCTC AGGCCAAAAA
    AGTTCGTGTT CTTGAGCTTT CTCGCAGGCA ATACATTTTT CCTCTGTTTT
    TTTTTTGGTT TTCAAAAAAA AAAAATTATT TGGTTTGTCC CTTTAAAACC
    TTTCTAATTT GGGCAAAATT ACGTGATGAT AATTTTGATG TTAAACTTCT
    AAAAAAACAA ATTTTTTTGT TTCAAAAATA TTTTAAGAAA AATTGATGTC
    TTCTATCTCA TTTTACCCAA CCCCAATTTA CAAAATTTGA TTTTGAAAAA
    TTGCCAAAAT TTTCTTTTTC TTGAACCTTT TTTACTGAAC GTGACTTAAA
    CAGGGATGCT TGATCAAAAA AAACGTCCCC TTTGTGCATA TTTAATGTGA
    AAATCTCTAA AAAAATCTAA TTCTTTTTTT GTTGTTGAAA AATCCCCAAA
    AAATGGTAAA AATGTATATA ATTGTATATT TTTTCAAAAT TTTGTT

AT3G47340.1

Model type
Protein coding
Short Description
glutamine-dependent asparagine synthase 1
Curator Summary
encodes a glutamine-dependent asparagine synthetase, the predicted ASN1 peptide contains a purF-type glutamine-binding domain, and is expressed predominantly in shoot tissues, where light has a negative effect on its mRNA accumulation. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
glutamine-dependent asparagine synthase 1 (ASN1); CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Asparagine synthase (InterPro:IPR001962), Asparagine synthase, glutamine-hydrolyzing (InterPro:IPR006426), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: asparagine synthetase 2 (TAIR:AT5G65010.1); Has 10466 Blast hits to 10069 proteins in 1927 species: Archae - 313; Bacteria - 6097; Metazoa - 215; Fungi - 282; Plants - 342; Viruses - 7; Other Eukaryotes - 3210 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G47340.2

Model type
Protein coding
Short Description
glutamine-dependent asparagine synthase 1
Curator Summary
encodes a glutamine-dependent asparagine synthetase, the predicted ASN1 peptide contains a purF-type glutamine-binding domain, and is expressed predominantly in shoot tissues, where light has a negative effect on its mRNA accumulation. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
glutamine-dependent asparagine synthase 1 (ASN1); CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Asparagine synthase (InterPro:IPR001962), Asparagine synthase, glutamine-hydrolyzing (InterPro:IPR006426), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: asparagine synthetase 2 (TAIR:AT5G65010.2); Has 10371 Blast hits to 9977 proteins in 1906 species: Archae - 313; Bacteria - 6018; Metazoa - 215; Fungi - 282; Plants - 337; Viruses - 5; Other Eukaryotes - 3201 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G47340.3

Model type
Protein coding
Short Description
glutamine-dependent asparagine synthase 1
Curator Summary
encodes a glutamine-dependent asparagine synthetase, the predicted ASN1 peptide contains a purF-type glutamine-binding domain, and is expressed predominantly in shoot tissues, where light has a negative effect on its mRNA accumulation. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
glutamine-dependent asparagine synthase 1 (ASN1); CONTAINS InterPro DOMAIN/s: Rossmann-like alpha/beta/alpha sandwich fold (InterPro:IPR014729), Asparagine synthase (InterPro:IPR001962), Asparagine synthase, glutamine-hydrolyzing (InterPro:IPR006426), Glutamine amidotransferase, type II (InterPro:IPR017932); BEST Arabidopsis thaliana protein match is: asparagine synthetase 3 (TAIR:AT5G10240.1); Has 9911 Blast hits to 9877 proteins in 1912 species: Archae - 315; Bacteria - 5703; Metazoa - 210; Fungi - 275; Plants - 333; Viruses - 5; Other Eukaryotes - 3070 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT3G47341.1

Model type
Protein coding
Computational Description
unknown protein; LOCATED IN: endomembrane system; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR