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GT18819

CSHL Arabidopsis genetrap mutant lines

Original: GT18819

Phenotypes

Morphological phenotypes

Original description

  • embryo lethal (unconfirmed)
Anatomical entity
/structure development stage		 Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)

Original: GT18819

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25574790 [Map]; Strand: c
      • AT5G63910.1 - [+] show detail - farnesylcysteine lyase
        Distance: -703; Region: promoter
      • AT5G63920.1 - [+] show detail - topoisomerase 3alpha
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: GT18819 - 5-prime edge 
    CCTACTTTCT CCCACACATA CAATAGAATT ACCTCGGGAA CGTGTTTCAT 
    GACCGGAGCT TGGTACAGAT CTGCCGGGTC GCAGGAGTGC CATTTGCGGT 
    AACGATCGGC GAACTCAAGC TCCATCAGGT GGCCGATGAC GGAGGTCATT 
    AGCATACGAC ACGGCTGCCC ATTGATGGCG TAATCGAACT CGAAGATTTT 
    GTTGTACCGA GACCTACCCT CCCGAGTCCG GAAAGTTCCA CGGGATAGAA 
    TCCCCGCCAC TGACTTCGCC ACGGACGGCT TCTCCGCCAC GTTCAACACT 
    GTCACGGGGC CACCGCCTCG TCGCGACATC GCCGGTAAAG AAAACCCTAA 
    CGGTTAGTAA TTACGTTTTT TTGTTTTCAG TAGGCGAATT TTCCCGCTCT 
    TATGATATAG CCTAGCTACT ATTGGTGGTG TCACTGAGAC ACTGACCGAA 
    CAGTTGTGGA AATTTGTGCC TTTAATGGGC TTCAATAATT CGGTTAAAGT 
    TGATCTCACA ATTGAAATTG AATCAAAACC GGACTCGGAT CCGGTTAGCT 
    TCTTATGATA TATGACTATA TGATAAGTAT GTTTAAATCG TATAGAATAT 
    AGATCTTGAG CTTAATGAGA TTAATTCAAT TTCTTTAGAA CACTTGCAAA 
    ATAAATAAAT AAAACAAAAT AATATTTTTC CTTTATGCAC TATAGAGTTA 
    GTCCGATAGG ATAAGGGCCC AAGAAGGCCT CATTGATAAT TTACAATCGA 
    GAGAGAGAGC GATAAAGTCA ACTCGTTGAC TAAAATGAA

AT5G63910.1

Model type
Protein coding
Short Description
farnesylcysteine lyase
Curator Summary
Encodes a farnesylcysteine lyase (EC 1.8.3.5) involved in a salvage /detoxification pathway of farnesylcysteine (FC) residues that are liberated during the degradation of prenylated proteins. Because FC is a competitive inhibitor of prenylcysteine methyltransferases involved in the down-regulation of ABA signaling, fcly mutants with elevated FC levels are hypersensitive to ABA. The protein also appears to be glycosylated when translated in vitro in the presence of microsomal membranes and it likely requires FAD for enzymatic activity.
Computational Description
farnesylcysteine lyase (FCLY); FUNCTIONS IN: prenylcysteine oxidase activity; INVOLVED IN: prenylated protein catabolic process, abscisic acid mediated signaling pathway, farnesyl diphosphate metabolic process; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Prenylcysteine lyase (InterPro:IPR010795), Prenylcysteine oxidase (InterPro:IPR017046); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G63920.1

Model type
Protein coding
Short Description
topoisomerase 3alpha
Curator Summary
Encodes topoisomerase 3alpha. Suppresses somatic crossovers. Essential for resolution of meiotic recombination intermediates.
Computational Description
topoisomerase 3alpha (TOP3A); FUNCTIONS IN: DNA topoisomerase activity, DNA topoisomerase type I activity, DNA binding, zinc ion binding, nucleic acid binding; INVOLVED IN: in 7 processes; LOCATED IN: chromosome; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: DNA topoisomerase, type IA, zn finger (InterPro:IPR013498), DNA topoisomerase, type IA, core (InterPro:IPR000380), DNA topoisomerase, type IA, domain 2 (InterPro:IPR003601), DNA topoisomerase, type IA, DNA-binding (InterPro:IPR003602), DNA topoisomerase, type IA, central (InterPro:IPR013497), Zinc finger, GRF-type (InterPro:IPR010666), DNA topoisomerase, type IA, central region, subdomain 3 (InterPro:IPR013826), Toprim domain, subgroup (InterPro:IPR006154), DNA topoisomerase, type IA, central region, subdomain 1 (InterPro:IPR013824), Toprim domain (InterPro:IPR006171), Zinc finger, CCHC-type (InterPro:IPR001878); BEST Arabidopsis thaliana protein match is: DNA topoisomerase, type IA, core (TAIR:AT2G32000.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR