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GT16566

CSHL Arabidopsis genetrap mutant lines

Original: GT16566

Phenotypes

Morphological phenotypes

Original description

  • embryo lethal (unconfirmed)
Anatomical entity
/structure development stage		 Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)

Original: GT16566

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 6136347 [Map]; Strand: c
    Flanking Genome Sequence: GT16566 - 3-prime edge 
    AGAGTATTTT ACCGACCGTT ACCGACCGTT TTCATCCCTA GGTAGGAGCT 
    TCAAGAACAA TCCTATCCCT ATCTCTATCT TCTTCCCTCT TCACCTTCTC 
    CAAAATCCCT CACGTTTTTC CATTTCTCCG CCTCCACAAA CCCAGATTCC 
    ACCACGCGTT TCGTCCTCTT TACTCCGCCG CCGCAACAAC

AT4G09730.1

Model type
Protein coding
Short Description
RH39
Curator Summary
Encodes RH39, a DEAD-box protein involved in the introduction of the hidden break into the 23S rRNA in the chloroplasts. Recombinant RH39 binds to the 23S rRNA in a segment adjacent to the stem-loop creating the hidden break target loop in a sequence-dependent manner. Has ATP-hydrolyzing activity at a Kcat of 5.3 /min in the presence of rRNA sequence. Mutants have drastically reduced level of level of ribulose 1,5-bisphosphate carboxylase/oxygenase.
Computational Description
RH39 (RH39); FUNCTIONS IN: LSU rRNA binding, ATPase activity, ATP-dependent helicase activity; INVOLVED IN: chloroplast ribulose bisphosphate carboxylase complex biogenesis, chloroplast rRNA processing; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: RNA helicase, DEAD-box type, Q motif (InterPro:IPR014014), DNA/RNA helicase, DEAD/DEAH box type, N-terminal (InterPro:IPR011545), DEAD-like helicase, N-terminal (InterPro:IPR014001), DNA/RNA helicase, C-terminal (InterPro:IPR001650), Helicase, superfamily 1/2, ATP-binding domain (InterPro:IPR014021); BEST Arabidopsis thaliana protein match is: DEA(D/H)-box RNA helicase family protein (TAIR:AT3G06980.1); Has 40425 Blast hits to 39698 proteins in 3032 species: Archae - 675; Bacteria - 19989; Metazoa - 5933; Fungi - 4498; Plants - 2407; Viruses - 9; Other Eukaryotes - 6914 (source: NCBI BLink).
Link
InterPro Scan - TAIR