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GT15829

CSHL Arabidopsis genetrap mutant lines

Original: GT15829

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Original: GT15829

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 2; Position: 14539250 [Map]; Strand: c
      • AT2G34490.1 - [+] show detail - cytochrome P450, family 710, subfamily A, polypeptide 2
        Distance: -1818; Region: intergenic
      • AT2G34500.1 - [+] show detail - cytochrome P450, family 710, subfamily A, polypeptide 1
        Distance: 229; Region: intergenic
    Flanking Genome Sequence: GT15829 - 3-prime edge 
    AAACGGTAGA GGATTTTACC GACCGTTACC GACCGTTTTC ATCCCTAGCT 
    ACACCCAAAG AATTTTCGAT GTGGAACCAA TTATCATAGC TAGACAAAAC 
    CCTGGATTTA TATCCCTAGA GATCTCGTGT GATAGAAATC TTGATTTGGT 
    ACAAAGAAAA ACAATAACAT GGTTCAAAAG TCTTTGTTTA AGTAGCACGA 
    AACCAATCAA TGATTTGATT GATGATTTGG GTAATGTAAT ACAAAATGGA 
    CGAATTGATC AGTTAACGAG AGTTACGGAC AAAGAATAAA GTCGGCAAGA 
    AAAAAAATAC AAGTATGAAA CACGTTGTAA CATCACTGAT GTTCACTGGA 
    AGACAACACA AAACAAATAT GGTGAAGACA TGGCAAAACA AAGACAAGTC 
    TTCTCAAATT CAATAAGACA AGAACACTCG TGGGCCACTC ACTTCGAGAG 
    ACACATCACG AAAAGTGATA AATTCACACT GGTTTCTTTG ACCACCAAGA 
    ATAAATTTAA GAAAAATCCA AAAAATTTAG GAAAAGTTGG GATACTTTGC 
    GACGCGCCTA GACAAGAACA CGGTGCACCC ATCCTTGGGC GATATAGTAG 
    GGCAGTACAC GATCTCATCA CAACCGTCTG ATCGAAGCCT CTTGAAATCC 
    AACAACGACG AGAACATTGC AATGAAGAGT ACGAGGTGGT TCAA

AT2G34490.1

Model type
Protein coding
Short Description
cytochrome P450, family 710, subfamily A, polypeptide 2
Curator Summary
Encodes a protein with C22-sterol desaturase activity. The enzyme was shown to catalyze the conversion of both 24-epi-campesterol and β-sitosterol to brassicasterol and stigmasterol, respectively, in the presence of NADPH.
Computational Description
cytochrome P450, family 710, subfamily A, polypeptide 2 (CYP710A2); FUNCTIONS IN: C-22 sterol desaturase activity, oxygen binding; INVOLVED IN: oxidation reduction; LOCATED IN: endomembrane system; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, conserved site (InterPro:IPR017972), Cytochrome P450, E-class, group I (InterPro:IPR002401); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 710, subfamily A, polypeptide 1 (TAIR:AT2G34500.1); Has 26372 Blast hits to 26256 proteins in 1476 species: Archae - 47; Bacteria - 2960; Metazoa - 10471; Fungi - 4624; Plants - 7423; Viruses - 0; Other Eukaryotes - 847 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT2G34500.1

Model type
Protein coding
Short Description
cytochrome P450, family 710, subfamily A, polypeptide 1
Curator Summary
Encodes a protein with C22-sterol desaturase activity. The enzyme was shown to catalyze in the presence of NADPH the conversion of β-sitosterol to stigmasterol, but not that of 24-epi-campesterol to brassicasterol (unlike CYP710A2).
Computational Description
cytochrome P450, family 710, subfamily A, polypeptide 1 (CYP710A1); FUNCTIONS IN: C-22 sterol desaturase activity, oxygen binding; INVOLVED IN: oxidation reduction; LOCATED IN: endomembrane system; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: LP.04 four leaves visible, 4 anthesis, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, conserved site (InterPro:IPR017972), Cytochrome P450, E-class, group I (InterPro:IPR002401); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 710, subfamily A, polypeptide 2 (TAIR:AT2G34490.1); Has 25674 Blast hits to 25623 proteins in 1435 species: Archae - 46; Bacteria - 2777; Metazoa - 10394; Fungi - 4411; Plants - 7239; Viruses - 0; Other Eukaryotes - 807 (source: NCBI BLink).
Link
InterPro Scan - TAIR