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GT14588

CSHL Arabidopsis genetrap mutant lines

Original: GT14588

Phenotypes

Morphological phenotypes

Original description

  • embryo lethal (unconfirmed)
Anatomical entity
/structure development stage		 Phenotypes
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)

Original: GT14588

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 1327843 [Map]; Strand: c
      • AT5G04610.1 - [+] show detail - S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
        Distance: -915; Region: promoter
      • AT5G04620.1 - [+] show detail - biotin F
        Distance: 0; Region: exon1 (5UTR)
      • AT5G04620.2 - [+] show detail - biotin F
        Distance: 0; Region: exon2 (CDS)
    Flanking Genome Sequence: GT14588 - 5-prime edge 
    TTTCTCCCTG TTTGCAGCAG CGTTTGATAT TGTAGGATGT GAGCTCAAAC 
    CCAAATAATC ATTCCCAGAG AACAAAAGCA GCTTCTTGAA TCTCCCTTTT 
    CTACACTCAG CTAATGCATC TCCACTAAAA ATCTCTTCTC CTGTCAACGT 
    GAAACTGATA CAACATAAAA ACCCAGTCTT TTGCCTAATC AATTCAGCTT 
    CTTCACTAAA CCCAAATTAT AACCATCAAT TACACTTTGC ATAAAGCCAT 
    AATTCACTGT CTAAAGTATG AAAACGAAAT CGCATTTGAG TTCAAAGGAA 
    GAAAAAAACA CCAAACTTTG GAAACTCCGA AACTCTCGGC AAAAAAAAAT 
    ATCAGGAAAG AAATGAAACG TACCGTTGCT GGGTTCATCG TGAAGCCATT 
    TCTGAAATGT AGGAATCGAG ACAGACACCT CAACTGAAGT CCGATCCCAT 
    TGACACAAAC CGTCAAACAC CTCGAACCCG TCTCCTCCAT TGGCTCTGCT 
    TTTCACTATT TCTTCTTCGT TTTGCCAAGA CATGCAAATG GGCCTCAAAG 
    ATCGAAGAAT TTGCCTGGAT TCAAGCACAT TCACTGCTTC TTCCACAGTT 
    TTATCCCACG AATGATCCGC CATTCTAAGT GCAAACAGAA TTGTGATTTG 
    GGCGAATTTT AAAAAGTGGT TCGGT

AT5G04610.1

Model type
Protein coding
Short Description
S-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Computational Description
S-adenosyl-L-methionine-dependent methyltransferases superfamily protein; FUNCTIONS IN: catalytic activity; EXPRESSED IN: shoot apex, sperm cell, flower; EXPRESSED DURING: petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: Spermine synthase (InterPro:IPR001045); BEST Arabidopsis thaliana protein match is: S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (TAIR:AT2G31740.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G04620.1

Model type
Protein coding
Short Description
biotin F
Curator Summary
The cDNA encoding 7-keto-8-aminopelargonic acid (KAPA) synthase, the first committed enzyme of the biotin synthesis pathway has been cloned and its molecular function confirmed (functional complementation of an E. coli mutant). The subcellular localization of the enzyme (cytosol) proves that the biotin biosynthesis in plants takes place in different compartments which differs from the biosynthetic route found in microorganisms.
Computational Description
biotin F (BIOF); CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: long chain base2 (TAIR:AT5G23670.2); Has 10202 Blast hits to 10184 proteins in 2138 species: Archae - 77; Bacteria - 6906; Metazoa - 648; Fungi - 572; Plants - 222; Viruses - 9; Other Eukaryotes - 1768 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G04620.2

Model type
Protein coding
Short Description
biotin F
Curator Summary
The cDNA encoding 7-keto-8-aminopelargonic acid (KAPA) synthase, the first committed enzyme of the biotin synthesis pathway has been cloned and its molecular function confirmed (functional complementation of an E. coli mutant). The subcellular localization of the enzyme (cytosol) proves that the biotin biosynthesis in plants takes place in different compartments which differs from the biosynthetic route found in microorganisms.
Computational Description
biotin F (BIOF); CONTAINS InterPro DOMAIN/s: Aminotransferase, class I/classII (InterPro:IPR004839), Pyridoxal phosphate-dependent transferase, major domain (InterPro:IPR015424), Pyridoxal phosphate-dependent transferase, major region, subdomain 1 (InterPro:IPR015421); BEST Arabidopsis thaliana protein match is: long chain base2 (TAIR:AT5G23670.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR