F27109

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F27109

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F27109

Determination of introduced cDNA(s)

  • RAFL09-06-N13 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-06-N13 - Resource: pda17616

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-06-N13 - full reading 
    CCCGAACCTA GAAATCGAAA ATATCAGATC TAAAGAAGAG CGTCTTCATA 
    AACGCCCTTC GTCTTCTTCC CTGCAAATCT ATTTTCTAGA TCTTTCTTTC 
    TTCCGCGAAA CGACAATGAG AGAGTGCATT TCGATCCACA TTGGTCAGGC 
    TGGTATTCAA GTCGGAAATG CTTGCTGGGA GCTTTACTGT CTTGAACATG 
    GCATTCAGCC TGATGGCCAG ATGCCTGGTG ACAAGACTGT TTGGTGGAGG 
    TGATGATGCT TTCAACACCT TCTTCAGTGA AACCGGTGCA GGTAAGCACG 
    TCCCACGTGC TGTCTTTGTT GATCTTGAGC CAACTGTGAT CGACGAGGTC 
    AGGACTGGTA CTTACCGTCA GCTTTTCCAC CCTGAACAAC TCATCAGTGG 
    TAAAGAAGAC GCTGCTAACA ATTTCGCCCG TGGTCATTAC ACCATTGGGA 
    AAGAGATTGT TGATCTCTGC TTGGACCGGA TCAGAAAGCT CGCTGATAAC 
    TGTACTGGTC TTCAAGGATT CCTTGTTTTC AACGCTGTTG GTGGTGGGAC 
    TGGATCTGGT CTTGGATCTC TTCTCCTTGA GAGACTTTCT GTTGACTATG 
    GGAAAAAGTC CAAGTTGGGT TTCACTGTTT ACCCATCTCC ACAGGTGTCT 
    ACCTCTGTGG TTGAGCCTTA CAACAGTGTC CTCTCCACTC ACTCTCTCTT 
    GGAACACACT GATGTCTCCA TCCTCCTCGA CAATGAAGCT ATCTATGACA 
    TCTGTAGACG CTCCCTTAAC ATTGAGAGAC CTACCTACAC CAACCTCAAC 
    CGTCTCGTCT CTCAGGTTAT TTCTTCCTTG ACTGCTTCTC TGAGGTTCGA 
    TGGTGCCTTG AATGTTGATG TTACTGAGTT CCAAACCAAC TTGGTTCCAT 
    ACCCAAGGAT CCACTTCATG CTTTCGTCCT ATGCACCAGT CATCTCCGCG 
    GAGAAGGCCT TCCATGAGCA ACTCTCAGTT GCTGAGATCA CGAACAGTGC 
    CTTTGAACCT GCTTCCATGA TGGCAAAGTG TGACCCGCGA CATGGAAAGT 
    ACATGGCTTG CTGTTTGATG TACCGTGGTG ATGTAGTCCC CAAGGATGTG 
    AACGCAGCGG TTGGCACCAT CAAGACCAAG CGAACTATTC AGTTTGTTGA 
    CTGGTGTCCT ACTGGATTCA AGTGTGGTAT CAACTACCAG CCACCAACTG 
    TTGTTCCTGG AGGTGATCTT GCTAAAGTCC AGAGAGCTGT TTGTATGATC 
    TCAAACTCAA CCAGTGTTGC TGAGGTATTC TCCCGTATTG ATCACAAGTT 
    TGATCTTATG TACGCCAAAC GTGCTTTCGT TCACTGGTAT GTTGGTGAGG 
    GTATGGAAGA AGGAGAATTC TCAGAGGCTC GTGAGGATCT TGCAGCATTG 
    GAGAAGGATT ATGAAGAGGT CGGTGCTGAA GGTGGTGACG ATGAGGATGA 
    TGAAGGAGAG GAATACTAAG AAGAATGTTT CTTAAAAATT GGATTTTGTG 
    TTGGGTTTTT CTCTATTATC TCGTGTTGTG ATGAATGGGG GCTCGAAACT 
    CTTACGAGTC TTAGTTGTGT GTTTTTCTAA ACCTATATTT CTATCTCTTG 
    CTGGTGGTTT GATTTCTATT ATCTAGTTCG TTTGTGTATT GACTCATGTT 
    TCTGAAAAAA AAAA

    Gene models with high sequence identity

    1. AT4G14960.2 [+] show detail - Tubulin/FtsZ family protein
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Alpha tubulin 1.7e-109
    FPrintScan Tubulin 5e-81
    FPrintScan Tubulin 5e-81
    FPrintScan Tubulin 5e-81
    FPrintScan Tubulin 5e-81
    FPrintScan Tubulin 5e-81
    FPrintScan Tubulin 5e-81
    FPrintScan Tubulin 5e-81
    FPrintScan Tubulin 5e-81
    Coil coiled-coil 0
    PatternScan Tubulin, conserved site 0
    HMMPanther Alpha tubulin 7.1e-236
    HMMPanther Tubulin 7.1e-236
    HMMSmart no description 1.4e-73
    HMMSmart Tubulin/FtsZ, 2-layer sandwich domain 1.7e-52
    Gene3D Tubulin/FtsZ, GTPase domain 1.3e-12
    Gene3D Tubulin/FtsZ, GTPase domain 9.7e-97
    Gene3D Tubulin/FtsZ, 2-layer sandwich domain 1.2e-47
    Gene3D no description 1.1e-21
    superfamily Tubulin/FtsZ, N-terminal 3.6e-13
    superfamily Tubulin/FtsZ, C-terminal 3.7e-83
    superfamily Tubulin/FtsZ, N-terminal 2e-82
    HMMPfam Tubulin/FtsZ, GTPase domain 1.2e-08
    HMMPfam Tubulin/FtsZ, GTPase domain 4.4e-57
    HMMPfam Tubulin/FtsZ, 2-layer sandwich domain 1.3e-50

    See the detailed result >

    AT4G14960.2

    Model type
    Protein coding
    Short Description
    Tubulin/FtsZ family protein
    Curator Summary
    Encodes an alpha-tubulin isoform required for right handed helical growth.
    Computational Description
    TUA6; FUNCTIONS IN: protein binding, structural constituent of cytoskeleton; INVOLVED IN: response to salt stress, microtubule cytoskeleton organization, cellular response to gravity; LOCATED IN: in 8 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Alpha tubulin (InterPro:IPR002452), Tubulin (InterPro:IPR000217), Tubulin/FtsZ, GTPase domain (InterPro:IPR003008), Tubulin/FtsZ, N-terminal (InterPro:IPR019746), Tubulin/FtsZ, C-terminal (InterPro:IPR008280), Tubulin, conserved site (InterPro:IPR017975), Tubulin/FtsZ, 2-layer sandwich domain (InterPro:IPR018316); BEST Arabidopsis thaliana protein match is: tubulin alpha-2 chain (TAIR:AT1G50010.1); Has 22664 Blast hits to 22566 proteins in 4681 species: Archae - 4; Bacteria - 25; Metazoa - 4398; Fungi - 13427; Plants - 1532; Viruses - 0; Other Eukaryotes - 3278 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR