F23926

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F23926

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1 rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F23926

Determination of introduced cDNA(s)

  • RAFL14-96-E19 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL14-96-E19 - Resource: pda12548

    full reading [+] show sequence

    Nucleotide sequence: RAFL14-96-E19 - full reading 
    GATCGTCTTC TCTTTCCCTA AACACACTGA TTATTTTCTC TCCGACGCCG 
    CCATGTCTCT GCTCTCAGAT CTCGTTAACC TCAACCTCAC CGATGCCACC 
    GGGAAAATCA TCGCCGAATA CATATGGATC GGTGGATCTG GAATGGATAT 
    CAGAAGCAAA GCCAGGACAC TACCAGGACC AGTGACTGAT CCATCAAAGC 
    TTCCCAAGTG GAACTACGAC GGATCCAGCA CCGGTCAGGC TGCTGGAGAA 
    GACAGTGAAG TCATTCTATA CCCTCAGGCA ATATTCAAGG ATCCCTTCAG 
    GAAAGACAAC AACATCCTGG TGATGTGTGA TGCTTACACA CCAGCTGGTG 
    ATCCTATTCC AACCAACAAG AGGCACAACG CTGCTAAGAT CTTCAGCCAC 
    CCCGACGTTG CCAAGGAGGA GCCTTGGTAT GGGATTGAGC AAGAATACAC 
    TTTGATGCAA AAGGATGTGA ACTGGCCAAT TGGTTGGCCT GTTGGTGGCT 
    ACCCTGGCCC TCAGGGACCT TACTACTGTG GTGTGGGAGC TGACAAAGCC 
    ATTGGTCGTG ACATTGTGGA TGCTCACTAC AAGGCCTGTC TTTACGCCGG 
    TATTGGTATT TCTGGTATCA ATGGAGAAGT CATGCCAGGC CAGTGGGAGT 
    TCCAAGTCGG CCCTGTTGAG GGTATTAGTT CTGGTGATCA AGTCTGGGTT 
    GCTCGATACC TTCTCGAGAG GATCACTGAG ATCTCTGGTG TAATTGTCAG 
    CTTCGACCCG AAACCAGTCC CGGGTGACTG GAATGGAGCT GGAGCTCACT 
    GCAACTACAG CACTAAGACA ATGAGAAACG ATGGAGGATT AGAAGTGATC 
    AAGAAAGCGA TAGGGAAGCT TCAGCTGAAA CACAAAGAAC ACATTGCTGC 
    TTACGGTGAA GGAAACGAGC GTCGTCTCAC TGGAAAGCAC GAAACCGCAG 
    ACATCAACAC ATTCTCTTGG GGAGTCGCGA ACCGTGGAGC GTCAGTGAGA 
    GTGGGACGTG ACACAGAGAA GGAAGGTAAA GGGTACTTCG AAGACAGAAG 
    GCCAGCTTCT AACATGGATC CTTACGTTGT CACCTCCATG ATCGCTGAGA 
    CGACCATACT CGGTTGATGA CACATTTCAT GATTTGATTT CTCTCCAATT 
    TGGTTTTTTT TTTTTCCCTT TTGATTGCAC TTTTCGATAA TAAAAAAATA 
    ATTCTTATTA TGGGCGTATT GTTGTGACAT TTTGTGTTTT GTTTCGAATA 
    ATTAAATAAG CGCTTCTTAA GGTGAAAATA AAAAAAAAAA AAAAAAAGAA 
    AAAAAAAAAA AAA

    Gene models with high sequence identity

    1. AT3G17820.1 [+] show detail - glutamine synthetase 1.3
      E-value: 0; Score: 99.92

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther GLUTAMINE SYNTHETASE (GLUTAMATE--AMMONIA LIGA 9e-258
    HMMPanther GLUTAMINE SYNTHETASE 9e-258
    PatternScan Glutamine synthetase, beta-Grasp 0
    PatternScan Glutamine synthetase, catalytic domain 0
    Gene3D Glutamine synthetase/guanido kinase, catalytic domain 5.4e-58
    HMMPfam Glutamine synthetase, catalytic domain 2.7e-61
    HMMPfam Glutamine synthetase, beta-Grasp 9.3e-19
    superfamily Glutamine synthetase/guanido kinase 1.3e-68
    superfamily Glutamine synthetase, beta-Grasp 6.9e-18

    See the detailed result >

    AT3G17820.1

    Model type
    Protein coding
    Short Description
    glutamine synthetase 1.3
    Curator Summary
    encodes a cytosolic glutamine synthetase, the enzyme has low affinity with substrate ammonium
    Computational Description
    glutamine synthetase 1.3 (GLN1.3); FUNCTIONS IN: glutamate-ammonia ligase activity, copper ion binding; INVOLVED IN: in 6 processes; LOCATED IN: cytosol, cytosolic ribosome, chloroplast, plasma membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 17 growth stages; CONTAINS InterPro DOMAIN/s: Glutamine synthetase, catalytic domain (InterPro:IPR008146), Glutamine synthetase, beta-Grasp (InterPro:IPR008147), Glutamine synthetase/guanido kinase, catalytic domain (InterPro:IPR014746); BEST Arabidopsis thaliana protein match is: glutamine synthase clone R1 (TAIR:AT5G37600.1); Has 10255 Blast hits to 10251 proteins in 3319 species: Archae - 147; Bacteria - 5273; Metazoa - 413; Fungi - 249; Plants - 1750; Viruses - 3; Other Eukaryotes - 2420 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR