F22930

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F22930

Determination of introduced cDNA(s)

RAFL09-06-B11 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-06-B11 - Resource: pda05054

full reading [+] show sequence

ATGCATTTTT AATCACTCCT CTTTCTAACT CCAATAAAAA CACAATAATG 
GATCTTCTTG TTATGCTCCT CTCTCTTCTC GTCTCTTATC TCATCTTCAA 
GATCTGGAAA CGCATAGACT CCAAGAGAGA CCAAAACTGC TACATCTTGG 
ACTACCAATG TCACAAACCT TCCGACGATC GTATGGTGAA CACTCAATTC 
AGCGGTGATA TCATCCTTCG AAACAAACAC CTTCGGCTCA ACGAGTACAA 
GTTCCTCCTC AAAGCCATCG TTAGTTCCGG TATCGGAGAG CAAACTTACG 
CACCAAGGCT CTTCTTTGAG GGCCGTGAAC AGCGTCCTAC GCTACAGGAT 
GGGCTCTCGG AGATGGAAGA GTTTTACATA GACACCATCG AGAAAGTCCT 
TAAGAGAAAC AAAATTTCAC CTTCGGAGAT TGACATCCTC GTAGTCAACG 
TCTCAATGCT CAATTCAACG CCTTCACTAT CTGCAAGAAT CATTAACCAT 
TACAAAATGA GAGAAGACAT AAAAGTCTTC AACTTGACGG CGATGGGATG 
TAGCGCGAGT GTTATCTCCA TAGATATTGT CAAAAACATC TTCAAAACAT 
ACAAGAACAA GCTTGCTCTT GTCGTGACCT CAGAGTCACT TAGTCCTAAC 
TGGTACAGCG GAAACAACCG GTCCATGATT CTTGCCAACT GTTTGTTCCG 
CTCTGGTGGC TGCGCTGTTC TCTTGACGAA CAAGCGTAGT CTGAGCAGGA 
GAGCCATGTT CAAGCTAAGG TGTTTGGTAA GGACGCACCA TGGAGCCAGA 
GACGATTCAT TCAACGCCTG TGTCCAAAAA GAAGACGAGC TAGGACATAT 
CGGTGTCCAC TTAGACAAGA CTCTTCCAAA AGCAGCGACT CGTGCTTTCA 
TAGACAACCT CAAAGTCATC ACTCCCAAGA TTCTTCCGGT AACAGAGCTT 
TTAAGGTTTA TGTTGTGTCT TCTTCTCAAG AAGCTTCGTA GCAGCCCTAG 
CAAAGGCTCG ACGAACGTGA CTCAGGCAGC TCCAAAGGCA GGGGTTAAAG 
CAGGGATTAA CTTCAAGACC GGTATTGACC ATTTCTGCAT TCACACTGGA 
GGTAAAGCGG TTATCGACGC GATCGGTTAC AGCCTCGACT TAAACGAGTA 
CGATCTTGAA CCGGCGAGGA TGACGTTACA CCGGTTTGGA AACACGTCAG 
CGAGTAGCTT GTGGTATGTT TTGGGGTATA TGGAGGCAAA GAAGAGGCTG 
AAGAGAGGAG ATAGAGTGTT CATGATCAGC TTTGGGGCAG GTTTCAAGTG 
CAATAGTTGC GTTTGGGAAG TTGTGAGAGA TCTTAACGTT GGAGAAGCTG 
TAGGGAATGT GTGGAATCAT TGCATTAATC AATACCCACC AAAATCGATT 
TTGAATCCGT TTTTCGAGAA GTATGGTTGG ATTCATGAAG AAGAAGATCC 
TGATACTTTC AAGATGCCCG AAGGATTTAT GTGAATGTTT TCTTGGGAAT 
CATTGTAAAC ATACATATAC AGTTACTTCA AACTGCAAAT CCCACAAAAG 
GTGACTATAT AGTTTATTTT CCTCTTTCTT TCTTTTATTT TATTTAATGT 
GGATTCTGAA TTGCTACATT TTACTCTCTC TTTCTTTCTT TTTCTTTTTT 
TCTAATTGGA TGGGAAAGAA AATATGAATG ACAAAGATAC TGCGATAT

Gene models with high sequence identity

AT1G07720.1 [+] show detail - 3-ketoacyl-CoA synthase 3
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
Gene3D	Thiolase-like, subgroup	1.4e-37
Gene3D	Thiolase-like, subgroup	3.6e-22
HMMPIR	Very-long-chain 3-ketoacyl-CoA synthase	1.3e-182
superfamily	Thiolase-like	2.5e-76
HMMPfam	FAE1/Type III polyketide synthase-like protein	2.1e-116
HMMPfam	3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal	8.8e-14

See the detailed result >

AT1G07720.1

Model type: Protein coding
Short Description: 3-ketoacyl-CoA synthase 3
Curator Summary: Encodes KCS3, a member of the 3-ketoacyl-CoA synthase family involved in the biosynthesis of VLCFA (very long chain fatty acids).
Computational Description: 3-ketoacyl-CoA synthase 3 (KCS3); FUNCTIONS IN: transferase activity, transferring acyl groups other than amino-acyl groups, catalytic activity, acyltransferase activity; INVOLVED IN: response to cold, response to light stimulus, response to osmotic stress; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Thiolase-like (InterPro:IPR016039), Very-long-chain 3-ketoacyl-CoA synthase (InterPro:IPR012392), 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal (InterPro:IPR013747), FAE1/Type III polyketide synthase-like protein (InterPro:IPR013601), Thiolase-like, subgroup (InterPro:IPR016038); BEST Arabidopsis thaliana protein match is: 3-ketoacyl-CoA synthase 12 (TAIR:AT2G28630.1); Has 2897 Blast hits to 2863 proteins in 795 species: Archae - 0; Bacteria - 1421; Metazoa - 0; Fungi - 2; Plants - 1311; Viruses - 0; Other Eukaryotes - 163 (source: NCBI BLink).
Link: InterPro Scan - TAIR