F20801

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F20801

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Original: F20801

Determination of introduced cDNA(s)

  • RAFL09-31-C01 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-31-C01 - Resource: pda05828

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-31-C01 - full reading 
    AAGTTTGAGA TCGGTGCGTT ATTGGATCTT ATTCAAAGAC ATAGAGTGAC 
    AATAGCGGCG CTTGTACCGC CGCTAGTGAT CGCTCTAGCT AAAAACCCGA 
    CGGTTAATTC TTATGATCTC TCCTCCGTTA GATTCGTTCT TTCCGGTGCA 
    GCTCCTTTAG GTAAAGAACT TCAAGATAGT CTTCGTCGCC GTCTCCCTCA 
    AGCCATCCTT GGCCAGGGAT ATGGTATGAC GGAGGCAGGA CCAGTGTTGT 
    CAATGAGCCT TGGGTTCGCT AAAGAACCGA TACCGACAAA ATCAGGTTCA 
    TGTGGGACTG TGGTCCGAAA CGCAGAGCTT AAAGTGGTTC ACCTTGAGAC 
    ACGTCTCTCT CTTGGATACA ACCAACCTGG TGAGATTTGT ATCCGCGGTC 
    AACAGATCAT GAAAGAGTAC TTGAACGATC CAGAAGCCAC TTCAGCAACA 
    ATAGACGAAG AAGGTTGGCT TCACACAGGA GACATTGGAT ATGTTGATGA 
    AGATGATGAG ATTTTCATTG TTGATCGACT TAAAGAAGTC ATCAAGTTCA 
    AAGGCTTTCA GGTCCCTCCA GCTGAGCTAG AGAGTTTGCT AATCAATCAC 
    CATTCAATTG CCGATGCAGC TGTTGTTCCG CAAAATGATG AAGTCGCTGG 
    GGAAGTTCCG GTGGCTTTCG TGGTGCGATC AAATGGAAAT GATATCACTG 
    AAGAAGATGT TAAAGAATAT GTTGCCAAAC AGGTGGTGTT CTATAAGAGA 
    TTGCACAAGG TCTTCTTTGT TGCTTCCATT CCTAAATCTC CTTCGGGAAA 
    AATACTAAGA AAGGACCTCA AAGCTAAGCT TTGTTGAATG TAGTTCTTTT 
    TAATATATTC CATTGATGAT TTAAACGTTC ATTTGTATCC GC

    Gene models with high sequence identity

    1. AT1G65060.1 [+] show detail - 4-coumarate:CoA ligase 3
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther 4-COUMARATE--COA LIGASE 6.1e-172
    HMMPanther ATP-DEPENDENT AMP-BINDING ENZYME FAMILY MEMBE 6.1e-172
    Gene3D no description 6.2e-23
    Gene3D no description 1e-21
    Gene3D no description 9.1e-26
    HMMPfam AMP-dependent synthetase/ligase 3.3e-63
    superfamily Acetyl-CoA synthetase-like 2.8e-92

    See the detailed result >

    AT1G65060.1

    Model type
    Protein coding
    Short Description
    4-coumarate:CoA ligase 3
    Curator Summary
    encodes an isoform of 4-coumarate:CoA ligase (4CL), which is involved in the last step of the general phenylpropanoid pathway. mRNA levels are not induced in response to wounding or to fungal infection by P. parasitica. mRNA is expressed in flowers, to a lesser degree in mature leaves and siliques and marginally in seedling roots and bolting stems of mature plants. The catalytic efficiency was in the following (descending) order: p-coumaric acid, caffeic acid, ferulic acid, cinnamic acid and 5-OH-ferulic acid. At4CL3 was unable to use sinapic acid as substrate.
    Computational Description
    4-coumarate:CoA ligase 3 (4CL3); CONTAINS InterPro DOMAIN/s: AMP-binding, conserved site (InterPro:IPR020845), AMP-dependent synthetase/ligase (InterPro:IPR000873); BEST Arabidopsis thaliana protein match is: 4-coumarate:CoA ligase 2 (TAIR:AT3G21240.1); Has 85516 Blast hits to 77968 proteins in 3765 species: Archae - 1155; Bacteria - 54010; Metazoa - 3516; Fungi - 4668; Plants - 2834; Viruses - 1; Other Eukaryotes - 19332 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR