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54-2726-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25574622 [Map]; Strand: c
      • AT5G63910.1 - [+] show detail - farnesylcysteine lyase
        Distance: -535; Region: promoter
      • AT5G63920.1 - [+] show detail - topoisomerase 3alpha
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 54-2726-1 - 5-prime edge 
    CCATTGATGG CGTAATCGAA CTCGAAGATT TTGTTGTACC GAGACCTACC 
    CTCCCGAGTC CGGAAAGTTC CACGGGATAG AATCCCCGCC ACTGACTTCG 
    CCACGGACGG CTTCTCCGCC ACGTTCAACA CTGTCACGGG GCCACCGCCT 
    CGTCGCGACA TCGCCGGTAA AGAAAACCCT AACGGTTAGT AATTACGTTT 
    TTTTGTTTTC AGTAGGCGAA TTTTCCCGCT CTTATGATAT AGCCTAGCAA 
    CTATTGGTGG TGTCACTGAG ACACTGACCG AACAGTTGTG GAAATTTGTG 
    CCTTTAATGG GCTTCAATAA TTCGGTTAAA GTTGATCTCA CAATTGAAAT 
    TGAATCAAAA CCGGACTCGG ATC

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25574738 [Map]; Strand: c
      • AT5G63910.1 - [+] show detail - farnesylcysteine lyase
        Distance: -651; Region: promoter
      • AT5G63920.1 - [+] show detail - topoisomerase 3alpha
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 54-2726-1 - 3-prime edge 
    ATCAATGGGC AGCCGTGTCG TATGCTAATG ACCTCCGTCA TCGGCCACCT 
    GATGGAGCTT GAGTTCGCCG ATCGTTACCG CAAATGGCAC TCCTGCGACC 
    CGGCAGATCT GTACCAAGCT CCGGTCATGA AACACAGTTC CCGAGGTAAT 
    TCTATTGTAT GTGTGTGCAC GCCTAATTGA TTTAATGTCC CATTGTACTT 
    GTATGCTTTT GTATAGTTCT GGGTTGTTGA TTTAGTCATT GCTTCTCTAA 
    CTAGGAATTG TTGATTGGGT CTGATTTGTT TTGGTAATTG CAACTGTATA 
    AAACGGGACT AGGATCTTTG ATTCGCCTTT ATAATGAAGT TGGGGTTTTC 
    TGACTTGCAA GTTTTGTTTA ATTCTGGTGT TGAGTGAATT AGGACAAAAA 
    GGATATTAAG AAGACATTGG AGGAGGAGGC AAGGAAAAGC GACTGGCTTG 
    TGCTGTGGCT TGATTGTGAT AGGGAAGGTG AAAACATTGC ATTTGAAGTT 
    GTAGATGTTT GCAGAGCTGT CAAGCATAAT CTTTTTATAC GCAGGGCTCA 
    TTTCTCTGCG TTAATTGACA GGTAGAGATT GCTTTTACAC GTTATTCCTC 
    ATATATCTTC TACTAAGCAC TCTATTTCAA ACGATTGAAC TGATTCCAAT 
    GATGTAATTG CTAGGGATAT TCATGAAGCA GTTCAGAATC TTAGAGATCC 
    TAATCAATN

AT5G63910.1

Model type
Protein coding
Short Description
farnesylcysteine lyase
Curator Summary
Encodes a farnesylcysteine lyase (EC 1.8.3.5) involved in a salvage /detoxification pathway of farnesylcysteine (FC) residues that are liberated during the degradation of prenylated proteins. Because FC is a competitive inhibitor of prenylcysteine methyltransferases involved in the down-regulation of ABA signaling, fcly mutants with elevated FC levels are hypersensitive to ABA. The protein also appears to be glycosylated when translated in vitro in the presence of microsomal membranes and it likely requires FAD for enzymatic activity.
Computational Description
farnesylcysteine lyase (FCLY); FUNCTIONS IN: prenylcysteine oxidase activity; INVOLVED IN: prenylated protein catabolic process, abscisic acid mediated signaling pathway, farnesyl diphosphate metabolic process; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Prenylcysteine lyase (InterPro:IPR010795), Prenylcysteine oxidase (InterPro:IPR017046); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G63920.1

Model type
Protein coding
Short Description
topoisomerase 3alpha
Curator Summary
Encodes topoisomerase 3alpha. Suppresses somatic crossovers. Essential for resolution of meiotic recombination intermediates.
Computational Description
topoisomerase 3alpha (TOP3A); FUNCTIONS IN: DNA topoisomerase activity, DNA topoisomerase type I activity, DNA binding, zinc ion binding, nucleic acid binding; INVOLVED IN: in 7 processes; LOCATED IN: chromosome; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: DNA topoisomerase, type IA, zn finger (InterPro:IPR013498), DNA topoisomerase, type IA, core (InterPro:IPR000380), DNA topoisomerase, type IA, domain 2 (InterPro:IPR003601), DNA topoisomerase, type IA, DNA-binding (InterPro:IPR003602), DNA topoisomerase, type IA, central (InterPro:IPR013497), Zinc finger, GRF-type (InterPro:IPR010666), DNA topoisomerase, type IA, central region, subdomain 3 (InterPro:IPR013826), Toprim domain, subgroup (InterPro:IPR006154), DNA topoisomerase, type IA, central region, subdomain 1 (InterPro:IPR013824), Toprim domain (InterPro:IPR006171), Zinc finger, CCHC-type (InterPro:IPR001878); BEST Arabidopsis thaliana protein match is: DNA topoisomerase, type IA, core (TAIR:AT2G32000.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR