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Mutant Lines
53-3205-1

53-3205-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence

Chromosome: 3; Position: 3335118 [Map]; Strand: w
- AT3G10660.1 - [+] show detail - calmodulin-domain protein kinase cdpk isoform 2
  Distance: -850; Region: promoter
- AT3G10670.1 - [+] show detail - non-intrinsic ABC protein 7
  Distance: 0; Region: exon6 (3UTR)

ATCATCCACT TAAAGTCTCA AACTTTATAA GAGCATTTAT AATAACAAAA 
TTTATTAACT GAATTTTAAA CTTGTTAGTT ACAAAAAGGT GTGGAATACT 
GTTCACATCC CAATAAAGTA GTTTGTCATG CAAATTGCAA ACCAAAACTA 
CGGGTCAAAG ATTGTAGTGA TAGATTTCAG TTTCCAATCA TCAGCTTTAG 
CTTCCATTGT ATTTGTCCCA TATAATAACC ATGGTCTCTC AAAGAAGGAA 
GACAGGTTAT ATGTATGATC CGCGATAACT AACCGGATAT CGCTTTGTAG 
CCTTCCTTTT CCAGTAGTTT GGCCAAGGAG TTGTCTCCGG TTTTAATGAT 
TCTCCCATTC TCCTGAAACC CAAGGAAACA TACATGAGCT TACCAAAATA 
TATATGATCA TTGTGTAGCC AGTAAAGAAG GCTGGTTTGG CAACAATGCT 
CACCATGATA TGTATGAGAG TTGGTTTGAT GTAGTCAAGT AGGCGTTGGT 
A

3 prime edge [+] show sequence
1. Chromosome: 3; Position: 3335007 [Map]; Strand: w
  - AT3G10660.1 - [+] show detail - calmodulin-domain protein kinase cdpk isoform 2
    Distance: -739; Region: promoter
Flanking Genome Sequence: 53-3205-1 - 3-prime edge
```
GTGAGTGGAT CAAATCCAAT CAAACCTAGT GGTTACTTAC ATTACTTGAG 
AAATTTGACA AAATAAAATT TAATTTGATT ATTCTATCGC CTGCTGCTGC 
TGGAACGAGT TGGTAATAGA AACAAATTCA TAATTTATGA CAAGAAGAAC 
GCAAAATTCG CTGATGATT
```

AT3G10660.1

Model type: Protein coding
Short Description: calmodulin-domain protein kinase cdpk isoform 2
Curator Summary: predicted to encode calcium-dependent protein kinase and is localized to the ER. Protein is myristoylated in a cell-free extract. Changing the proposed myristoylated site, G residue in the amino terminal, to A prevented the meristoylation . The G to A mutation decreased AtCPK2 membrane association by approximately 50%.
Computational Description: calmodulin-domain protein kinase cdpk isoform 2 (CPK2); CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand-like domain (InterPro:IPR011992), EF-hand (InterPro:IPR018248), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium dependent protein kinase 1 (TAIR:AT5G04870.1); Has 145304 Blast hits to 132515 proteins in 4265 species: Archae - 224; Bacteria - 17128; Metazoa - 53382; Fungi - 18632; Plants - 28672; Viruses - 609; Other Eukaryotes - 26657 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G10670.1

Model type: Protein coding
Short Description: non-intrinsic ABC protein 7
Curator Summary: Plastidic SufC-like ATP-binding cassette/ATPase essential for Arabidopsis embryogenesis. Involved in the biogenesis and/or repair of oxidatively damaged Fe
Computational Description: non-intrinsic ABC protein 7 (NAP7); FUNCTIONS IN: protein binding, ATPase activity, coupled to transmembrane movement of substances, transporter activity; INVOLVED IN: iron-sulfur cluster assembly, thylakoid membrane organization, embryo development ending in seed dormancy; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), ATPase SufC, SUF system FeS cluster assembly (InterPro:IPR010230), ABC transporter-like (InterPro:IPR003439), ABC transporter, conserved site (InterPro:IPR017871); BEST Arabidopsis thaliana protein match is: ABC transporter family protein (TAIR:AT4G33460.1); Has 256475 Blast hits to 243565 proteins in 3618 species: Archae - 5159; Bacteria - 209774; Metazoa - 3736; Fungi - 3348; Plants - 2780; Viruses - 4; Other Eukaryotes - 31674 (source: NCBI BLink).
Link: InterPro Scan - TAIR