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52-1178-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 6135700 [Map]; Strand: c
    Flanking Genome Sequence: 52-1178-1 - 3-prime edge 
    ACGAGGNTTT TACCTGACCG TTACCGACCG TTTTCATCCN TGCATGTTGG 
    TGGGTATTGT ACTATTGTTC ACTCAAGTAT ATGTATATGA CCATAAAAAA 
    AAAAAANGGG GTTTGNCNTN TGGGNCNNTT TTTTTTTTNA ANTTTNCCCC 
    AAATTTTNCC NNAAATNGGN AAAAAACCTT NTTCCANAAA ANNNNTAAAA 
    NNTTTTTTTN ATTTNTTTAA AAAAAAANNT TTATNGNTNN ANNCNNCNGG 
    NGTNNNTTNN NNCNNGGNTT NANTNTTTTT TAAAAAAAAA GNNTTNTNAA 
    AAAAAAAGGT TTTTNNCCAA ANNCNGGTTN NNNGGNNNNC NGGGTTTNAN 
    CGGGNTNTTN ANNCCCCCCC NTTTNCTAAT ANNAAAATTG GNGNNTTNNN 
    ATNNNAAAAA CNNNTNNCNC NNGNNAANTN CCNACCAANA CANCNNAANC 
    TNANCNACTG NTCNTAAAAA CTCTCGCCNG TTNTTTCCNA NTNCCNCCNN 
    NNTNTNTNAC CCTAATNGNG CCCCCNTNTG GGNCCTAAAA NGGTTTTGNN 
    NNNNGNNNNN ATTTANCCNC CNCCCTTGNA AAANACANAN TNTNNTCTNT 
    NNNNNCTNGN NNANAATNAA NCAACCCNTT CANAAAANNC CTNCNANTAN 
    NNCCNAAANC CNTTCNCTCN CCCNACCCAN TACNNNAAAT NCCCCCCNAN 
    NNNNCNGGGG NAGTTCCNGN GAGAANNAAN TNGCTTNNAN CTTTCNGNTN 
    TGTTTTTCNC GTGTCCNCAC NGNTCNNAAA NNTCCAGCNA NTTT

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 6135611 [Map]; Strand: c
    Flanking Genome Sequence: 52-1178-1 - 5-prime edge 
    CCAACATGAC CAATAGGCAA TAACCACTTA GGCTATAGCA AAAGAAAAGC 
    AAGGACAATG ATATCTCTCT TAGTTCTTCA AAGCTGTAAT GATTGCATTG 
    AAAGTATAAT TGCAGATTCA CTGAGTCCAC CACCTTCTTC TGCACGGATG 
    TAGCCTCCAG GTTGGAGATA GTACACTNGT AGCAGCTAGA ATCAGATCAC 
    GTCCGGGTAA TAATACAGCC TGAGAACTAA AGAAAATAGA CGACCATGCT 
    ATATCCTCTC TGAATTGTTT AAGACAATCT CTGTTTAGGT TTTGTCACCT 
    GAATAGCTAT TCGAGTGTAT TACATTCACA AAGTTTTTTA AAATATCACA 
    TGATCTAGGT TAAAGTTTGA CTTCAAAGTT TTACAATATT TCTATGAAAA 
    AAACTATACA TGCAAAATGA ACATATGGTC TAACTATAGA TGTAGTAACA 
    TATCCGACAG TTGAGTCAAA CCAAACTGAA ATAGTGTAGC ATGCAACTTT 
    ATATCGCCAT AGGGATTAAG AGCAATAATG GTTCTTCTTA CAAAAATAAA 
    ATTTGTATCC AGCAGAATCG ATTGATCATA CACAGACTTC TCCAATCAAT 
    TAATATATGA TGGAATGACA AAAAAATTGC TTCTACAAGC T

AT4G09730.1

Model type
Protein coding
Short Description
RH39
Curator Summary
Encodes RH39, a DEAD-box protein involved in the introduction of the hidden break into the 23S rRNA in the chloroplasts. Recombinant RH39 binds to the 23S rRNA in a segment adjacent to the stem-loop creating the hidden break target loop in a sequence-dependent manner. Has ATP-hydrolyzing activity at a Kcat of 5.3 /min in the presence of rRNA sequence. Mutants have drastically reduced level of level of ribulose 1,5-bisphosphate carboxylase/oxygenase.
Computational Description
RH39 (RH39); FUNCTIONS IN: LSU rRNA binding, ATPase activity, ATP-dependent helicase activity; INVOLVED IN: chloroplast ribulose bisphosphate carboxylase complex biogenesis, chloroplast rRNA processing; LOCATED IN: chloroplast; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: RNA helicase, DEAD-box type, Q motif (InterPro:IPR014014), DNA/RNA helicase, DEAD/DEAH box type, N-terminal (InterPro:IPR011545), DEAD-like helicase, N-terminal (InterPro:IPR014001), DNA/RNA helicase, C-terminal (InterPro:IPR001650), Helicase, superfamily 1/2, ATP-binding domain (InterPro:IPR014021); BEST Arabidopsis thaliana protein match is: DEA(D/H)-box RNA helicase family protein (TAIR:AT3G06980.1); Has 40425 Blast hits to 39698 proteins in 3032 species: Archae - 675; Bacteria - 19989; Metazoa - 5933; Fungi - 4498; Plants - 2407; Viruses - 9; Other Eukaryotes - 6914 (source: NCBI BLink).
Link
InterPro Scan - TAIR