1. Home
  2. Mutant Lines
  3. 52-0717-1

52-0717-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25573865 [Map]; Strand: c
      • AT5G63910.1 - [+] show detail - farnesylcysteine lyase
        Distance: 0; Region: exon1 (CDS)
      • AT5G63920.1 - [+] show detail - topoisomerase 3alpha
        Distance: -554; Region: promoter
    Flanking Genome Sequence: 52-0717-1 - 5-prime edge 
    GTGATAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
    CCCATCCTAC TTTCATCAAA TTGAGCGTCA CGAGATAGTC GGTGGACGCA 
    TGAGGACCGT CACTGTCGCC GGTGATACTT TCGAAGCCGG CGGTTCGATT 
    CTCCATCCGA AGAACTACCA CGTTAAGGAT TTCGTCGAAA GGTTCAATCT 
    GACNGGTTCG ATTACCGACG CCGATTGAAG AATCTTCAGC GATTGGAATC 
    TGGGATGGGA AGAGATTTGT CGTCAAGACA TTCGGTTCCG GTACCAAATT 
    TCCGTTTTTG GATACGATCG TCTCTTGGGT GAATGATCTC TACTTGTTCT 
    TACGTTATGG GTTATCACTG CTGAGAATGA GCAGCTTCAT TGAGGTATTT 
    AAATGTAGAA CCTTTTGATT TCAGAATTGG CTATCAATTG TTGAACAATG 
    AGTTTGTTGT TGTAAAGTGA TTTTAATTTG TTAGCTAGCA ATGAGGATGT 
    TTTCGTAACT TGATTTTGCA TCTAGGTTTG TGTTTGTGTG TGTGTGTGGA 
    TC

  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 25573868 [Map]; Strand: c
      • AT5G63910.1 - [+] show detail - farnesylcysteine lyase
        Distance: 0; Region: exon1 (CDS)
      • AT5G63920.1 - [+] show detail - topoisomerase 3alpha
        Distance: -551; Region: promoter
    Flanking Genome Sequence: 52-0717-1 - 3-prime edge 
    ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATCCA 
    AACATCAGGA TCTTGGCTTG ATTTAAGCCG GTGGAAACAG AGTAGTTACG 
    GAGGAAGTGA GCTACGGATG AGCCTCCGAT CCCACTCCCG ACGATACATA 
    CGGTCGCTGG AGAATCATCC TCGACGCCGC CGGTGGAATC GCCGGAGCAA 
    GGTAGAAGAA CCGGCGATAG TAGAGCGAAC AGAAGGGAGA TTGCTATCGG 
    GAAATCTTTC ATTTTAGTCA ACGAGTTGAC TTTATCGCTC TCTCTCTCGA 
    TTGTAAATTA TCAATGAGGC CTTCTTGGGC CCTTATCCTA TCGGACTAAC 
    TCTATAGTGC ATAAAGGAAA AATATTATTT TGTTTTATTT ATTTATTTTG 
    CAAGTGTTCT AAAGAAATTG AATTAATCTC ATTAAGCTCA AGATCTATAT 
    TCTATACGAT TTAAACATAC TTATCATATA GTCATATATC ATAAGAAGCT 
    AACCGGATC

AT5G63910.1

Model type
Protein coding
Short Description
farnesylcysteine lyase
Curator Summary
Encodes a farnesylcysteine lyase (EC 1.8.3.5) involved in a salvage /detoxification pathway of farnesylcysteine (FC) residues that are liberated during the degradation of prenylated proteins. Because FC is a competitive inhibitor of prenylcysteine methyltransferases involved in the down-regulation of ABA signaling, fcly mutants with elevated FC levels are hypersensitive to ABA. The protein also appears to be glycosylated when translated in vitro in the presence of microsomal membranes and it likely requires FAD for enzymatic activity.
Computational Description
farnesylcysteine lyase (FCLY); FUNCTIONS IN: prenylcysteine oxidase activity; INVOLVED IN: prenylated protein catabolic process, abscisic acid mediated signaling pathway, farnesyl diphosphate metabolic process; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Prenylcysteine lyase (InterPro:IPR010795), Prenylcysteine oxidase (InterPro:IPR017046); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G63920.1

Model type
Protein coding
Short Description
topoisomerase 3alpha
Curator Summary
Encodes topoisomerase 3alpha. Suppresses somatic crossovers. Essential for resolution of meiotic recombination intermediates.
Computational Description
topoisomerase 3alpha (TOP3A); FUNCTIONS IN: DNA topoisomerase activity, DNA topoisomerase type I activity, DNA binding, zinc ion binding, nucleic acid binding; INVOLVED IN: in 7 processes; LOCATED IN: chromosome; EXPRESSED IN: 17 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: DNA topoisomerase, type IA, zn finger (InterPro:IPR013498), DNA topoisomerase, type IA, core (InterPro:IPR000380), DNA topoisomerase, type IA, domain 2 (InterPro:IPR003601), DNA topoisomerase, type IA, DNA-binding (InterPro:IPR003602), DNA topoisomerase, type IA, central (InterPro:IPR013497), Zinc finger, GRF-type (InterPro:IPR010666), DNA topoisomerase, type IA, central region, subdomain 3 (InterPro:IPR013826), Toprim domain, subgroup (InterPro:IPR006154), DNA topoisomerase, type IA, central region, subdomain 1 (InterPro:IPR013824), Toprim domain (InterPro:IPR006171), Zinc finger, CCHC-type (InterPro:IPR001878); BEST Arabidopsis thaliana protein match is: DNA topoisomerase, type IA, core (TAIR:AT2G32000.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link
InterPro Scan - TAIR