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52-0713-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

Notice

More than one insertion position or no insertion point is expected.

  • 5 prime edge [+] show sequence

    1. Chromosome: 4; Position: 10931130 [Map]; Strand: c
      • AT4G20235.1 - [+] show detail - cytochrome P450, family 71, subfamily A, polypeptide 28
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 52-0713-1 - 5-prime edge 
    GNGAAAAACT AACAAAATCG GTTATACGAT AACGGTCGGT ACGGGATTTT 
    CCCATCCTAC TTTCATCCCT CCACCCTCAC CGCTCCTTTC GTTCCCTCAG 
    CCTCCGCCTA TGGTCCGCTC ATGCTCCTTC ACTTCGGTCG TGTCCCTACC 
    CTCGATTCTC GAAGTATATA TCATCCTCTG ACGTAGCTCA TGACGTCATG 
    AAAACACACN GATCTTAAGT TTGCCAACCG TCCCAAAACC AAAGCTGTCG 
    ATATAATTAT TAATGGTGGG AGAGATTGTT GAGATAAGCT TGAAATAAAA 
    CTTGAGATAA GTTATTTAGT TTAAAAGGAT TATGTTAAAT CTTTGGGAGA 
    TATCCAAAGA TATATTCTAG GAGTTATCTA GTATTAANCC TTATGNGTNT 
    ATGNACACAA GNNTGGTATA TATATGAGNG CNNAGNGNGG NAGACNANAN 
    GANNNNAGAG NGGGGNGGGN ANGGNNGNAA NNAAAGNNAG AGNANAAANN 
    NNNGNNNNNG NGGGGNANAA GANGAGGNNN NNNNNGNGNN NNNNNNNAGG 
    GNNANNG

  • 3 prime edge [+] show sequence

    1. Chromosome: 4; Position: 10945891 [Map]; Strand: c
      • AT4G20260.6 - [+] show detail - plasma-membrane associated cation-binding protein 1
        Distance: 2396; Region: intergenic
      • AT4G20270.1 - [+] show detail - Leucine-rich receptor-like protein kinase family protein
        Distance: -3770; Region: intergenic
    Flanking Genome Sequence: 52-0713-1 - 3-prime edge 
    ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCATTAC 
    TTAACACACA ACAATAGAAA TAAAAAGTAT AAAGTCCTAG GGTTTAGAAC 
    AGAGAAACAG AGTTGCTAGC AATAACTAGA TTTGTGAATT AAGAACTTAT 
    CTTCAAGGAC GAAATTTGAG CTCCGAAACA AAACTAGAGA TAGCNTTTAT 
    TACTCAGTTA GGACAATTTA AGAGTTTTTT TTTCTCTCTT TCAAATCGAA 
    TAGAGTTTAG TAATAGGACA ATAGGAGAAG ATTTTGACGC AAACCCAAAT 
    CGGATTCGCT CGGCCCAATT ATGAGGAATG TTTTTTTTCT TTTTCTTTTT 
    TTTTGGGNAN NCCGAAAANC ANNTTTTTTT TTAANANAAA NTTACNTTNC 
    NGTACCTNNN CAANTTAAAA NTTTTTAAAA AAANTNGAAN TTTTTTTNTT 
    ANGGAGGATN TNTNTNTTTG GNGGATTCNA AAAGTTTTTN TNTNGNAAAA 
    AAANCNGNGG GNANNGCGNA AAAANGTGGG CCCNTNGTTT TNTTTNAAAA 
    NGTNGNTTTT NTAAAAANGG GNNAANNGCC CCCCAGGTAA AGNCTCAAAC 
    CAAAAGGNNC AAAATTNTTA TTATNGCAAA CCAANTCCTT GAAAAAAANA 
    GACAAGGTGN ACCCCTTNGC CTTTTTACTA ATAAGGTTTT TCAANGAAAA 
    AAGTNNCCNC TCCGGNCCNT TAGGTCCNAA TGCAAAACCC NTNGCTGGCC 
    TTTTTCTGGC CNAAGGTTTG CTNTNAAACN CTTC

AT4G20235.1

Model type
Protein coding
Short Description
cytochrome P450, family 71, subfamily A, polypeptide 28
Computational Description
"cytochrome P450, family 71, subfamily A, polypeptide 28" (CYP71A28); FUNCTIONS IN: electron carrier activity, monooxygenase activity, iron ion binding, heme binding; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 13 plant structures; EXPRESSED DURING: 6 growth stages; CONTAINS InterPro DOMAIN/s: Cytochrome P450 (InterPro:IPR001128), Cytochrome P450, conserved site (InterPro:IPR017972); BEST Arabidopsis thaliana protein match is: cytochrome P450, family 71, subfamily A, polypeptide 14 (TAIR:AT5G24960.1); Has 11933 Blast hits to 8219 proteins in 526 species: Archae - 4; Bacteria - 111; Metazoa - 1259; Fungi - 469; Plants - 10011; Viruses - 0; Other Eukaryotes - 79 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT4G20260.6

Model type
Protein coding
Short Description
plasma-membrane associated cation-binding protein 1
Curator Summary
Encodes a Ca2+ and Cu2+ binding protein. N-terminal myristylation on glycine 2 appears to enable it to associate tightly with the plasma membrane. Recombinant PCaP1 interacts strongly with phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and PtdIns (3,4,5)P3, and weakly with PtdIns(3,5)P2 and PtdIns(4,5). It also interacts with calmodulin (CaM) in a calcium-dependent manner. CaM does not interfere with PCaP1 membrane localization but does weaken interactions between it and the PtdInsPs. PCaP1 has an apparent Kd of 10 uM for Cu2+ and can bind six ions per protein. Transcript levels for PCaP1 first fall and then rise following exposure to CuCl2. Mannitol, sorbitol, and the flg22 oligopeptide also increase expression levels.
Computational Description
plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation; LOCATED IN: plasma membrane; EXPRESSED IN: 34 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469).
Link
InterPro Scan - TAIR

AT4G20270.1

Model type
Protein coding
Short Description
Leucine-rich receptor-like protein kinase family protein
Curator Summary
Encodes a CLAVATA1-related receptor kinase-like protein required for both shoot and flower meristem function. It has a broad expression pattern and is involved in vascular strand development in the leaf, control of leaf shape, size and symmetry, male gametophyte development and ovule specification and function.
Computational Description
BARELY ANY MERISTEM 3 (BAM3); FUNCTIONS IN: protein serine/threonine kinase activity, protein kinase activity, ATP binding; INVOLVED IN: regulation of meristem growth, protein amino acid phosphorylation, transmembrane receptor protein tyrosine kinase signaling pathway, leaf development, floral organ development; LOCATED IN: endomembrane system; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, catalytic domain (InterPro:IPR000719), Leucine-rich repeat-containing N-terminal domain, type 2 (InterPro:IPR013210), Leucine-rich repeat (InterPro:IPR001611), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271); BEST Arabidopsis thaliana protein match is: Leucine-rich receptor-like protein kinase family protein (TAIR:AT5G65700.2); Has 219938 Blast hits to 139586 proteins in 4977 species: Archae - 151; Bacteria - 21929; Metazoa - 70277; Fungi - 10656; Plants - 90391; Viruses - 423; Other Eukaryotes - 26111 (source: NCBI BLink).
Link
InterPro Scan - TAIR