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52-0203-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 5; Position: 6836162 [Map]; Strand: c
      • AT5G20250.1 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon4 (CDS)
      • AT5G20250.2 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon5 (CDS)
      • AT5G20250.3 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon4 (CDS)
      • AT5G20250.4 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon5 (CDS)
    Flanking Genome Sequence: 52-0203-1 - 5-prime edge
    GTCTCCATTC CAGGTTGTTG GATCAGTGGA GGCCTCCGAT GTTGAATGCA 
    CATCACGACC ACGAATGGAG CCAGTGAGGC TATCAGTTTT AGTCTGGTGG
    AAAATGTTTT TTCTCTCTNT GCTACTCCAC TATGCTCCTT GGCAGTTATA
    CNCGCCAAGA ACTCCAGAGT ACTTGTTCAT GTTCCATATC TTTAGCAAGC
    TGAAACCAAA ACAAAGCATA TTAAGAAAAT GAGAAACCAA AAAGTTTTGA
    AGAATTATCT TTATAAGCCA AATTTGCTTA CCTGACACCA TCACGGGCAG
    GATCAGCGAA CAAACAATCG CGAGTTGGTC TACCANGGAG TCNAGCACGA
    ANAATCGATC CATCANGCA
  • 3 prime edge [+] show sequence

    1. Chromosome: 5; Position: 6836277 [Map]; Strand: c
      • AT5G20250.1 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon4 (CDS)
      • AT5G20250.2 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon5 (CDS)
      • AT5G20250.3 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon4 (CDS)
      • AT5G20250.4 - [+] show detail - Raffinose synthase family protein
        Distance: 0; Region: exon5 (CDS)
    Flanking Genome Sequence: 52-0203-1 - 3-prime edge
    GGTGGNGACT GTGCTGTTTA CTCCCAGAGT CAGAGGCGAA CTTATTGTTA 
    TGGCCATACA AGGTGTCTCT TCCAGTCTCA CTCAAAATCC GTGAGCACGA
    GATCTTCACG GTGAGCCCCA TTAGTCNTCT TGTTGATGGT GTATCTTTTG
    CCCCAATTGG TCTAGTANAC ATGTACAATT CGGGAGGAGC TATCGAAGGA
    CTTAGATATG AAGCCGAGAA GATGAAAGTG GTAATGGAAG TTAAAGGATG
    TGGCAAATTC GGATCTTACT CTTCTGTGAA GCCTAAGAGA TGCGTTGTTG
    AGTCAAATGA GATTGCATTC GAGTACGATT CCTCCTCTGG ATTGGTCACC
    TTTGAGTTAG ACAAAATGCC TATAGAGAAC AAACGAATTC ATCTGATCCA
    AGTTGA

AT5G20250.1

Model type
Protein coding
Short Description
Raffinose synthase family protein
Curator Summary
encodes a member of glycosyl hydrolase family 36. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
DARK INDUCIBLE 10 (DIN10); FUNCTIONS IN: hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: response to karrikin, aging, response to light stimulus, response to cold, response to sucrose stimulus; LOCATED IN: chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 2 (TAIR:AT3G57520.1); Has 568 Blast hits to 539 proteins in 163 species: Archae - 27; Bacteria - 128; Metazoa - 0; Fungi - 82; Plants - 321; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G20250.2

Model type
Protein coding
Short Description
Raffinose synthase family protein
Curator Summary
encodes a member of glycosyl hydrolase family 36. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
DARK INDUCIBLE 10 (DIN10); FUNCTIONS IN: hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: response to karrikin, aging, response to light stimulus, response to cold, response to sucrose stimulus; LOCATED IN: chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 2 (TAIR:AT3G57520.1); Has 568 Blast hits to 539 proteins in 163 species: Archae - 27; Bacteria - 128; Metazoa - 0; Fungi - 82; Plants - 321; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G20250.3

Model type
Protein coding
Short Description
Raffinose synthase family protein
Curator Summary
encodes a member of glycosyl hydrolase family 36. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
DARK INDUCIBLE 10 (DIN10); FUNCTIONS IN: hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: response to karrikin, aging, response to light stimulus, response to cold, response to sucrose stimulus; LOCATED IN: chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 2 (TAIR:AT3G57520.1); Has 568 Blast hits to 539 proteins in 163 species: Archae - 27; Bacteria - 128; Metazoa - 0; Fungi - 82; Plants - 321; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT5G20250.4

Model type
Protein coding
Short Description
Raffinose synthase family protein
Curator Summary
encodes a member of glycosyl hydrolase family 36. Expression is induced within 3 hours of dark treatment, in senescing leaves and treatment with exogenous photosynthesis inhibitor. Induction of gene expression was suppressed in excised leaves supplied with sugar. The authors suggest that the gene's expression pattern is responding to the level of sugar in the cell.
Computational Description
DARK INDUCIBLE 10 (DIN10); CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, catalytic core (InterPro:IPR017853), Raffinose synthase (InterPro:IPR008811); BEST Arabidopsis thaliana protein match is: seed imbibition 2 (TAIR:AT3G57520.1).
Link
InterPro Scan - TAIR