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Mutant Lines
13-2060-1

13-2060-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

3 prime edge [+] show sequence

Chromosome: 4; Position: 903128 [Map]; Strand: c
- AT4G02060.2 - [+] show detail - Minichromosome maintenance (MCM2/3/5) family protein
  Distance: 0; Region: exon10 (CDS)
- AT4G02060.1 - [+] show detail - Minichromosome maintenance (MCM2/3/5) family protein
  Distance: 0; Region: exon8 (CDS)

ATGAAAACGG TAGAGGTATT TTACCGACCG TTACCGACCG TTTTCAGTTC 
GAGCTGGCTT AGTAGCAGAC ACCTATCTGG AAGCAACGTC TGTTACTCAT 
TTCAAGAAGA AATATGAGGA GTAAGGCATC GTTACACCTT AACACTAACG 
AAATATTCTG ATTTAGTAAC CTATTCCGCG TTTTTTGCAT TTGCTTAACT 
TAAGAAGCAG CTTTGGTGTG TGCACAATAT CAGAAAGCAT CTTTATACCT 
TTTTTATTAT GATCTACAGA TATGAGTTTC AGAAAGATGA GGAAGAACAG 
ATTGCACGTT TGGCTGAGGA TGGTGACATA TACAATAAGC TGTCGCGATC 
CCTGGCCCCT GAGATTTATG GACATGAAGA TATTAAGAAA GCT

5 prime edge [+] show sequence
1. Chromosome: 4; Position: 903058 [Map]; Strand: c
  - AT4G02060.2 - [+] show detail - Minichromosome maintenance (MCM2/3/5) family protein
    Distance: 0; Region: exon10 (CDS)
  - AT4G02060.1 - [+] show detail - Minichromosome maintenance (MCM2/3/5) family protein
    Distance: 0; Region: exon8 (CDS)
Flanking Genome Sequence: 13-2060-1 - 5-prime edge
```
GCTCGAAGTG CCTTAAACCC AGTGTAAGGA ATGGGAAGAA AAATCCCTGA 
GAATT
```

AT4G02060.1

Model type: Protein coding
Short Description: Minichromosome maintenance (MCM2/3/5) family protein
Curator Summary: Member of the minichromosome maintenance complex, involved in DNA replication initiation. Abundant in proliferating and endocycling tissues. Localized in the nucleus during G1, S and G2 phases of the cell cycle, and are released into the cytoplasmic compartment during mitosis. Binds chromatin.
Computational Description: PROLIFERA (PRL); FUNCTIONS IN: protein binding, DNA-dependent ATPase activity, DNA binding, ATP binding; INVOLVED IN: sugar mediated signaling pathway, cell proliferation, DNA-dependent DNA replication initiation, DNA unwinding involved in replication; LOCATED IN: nuclear chromatin, nucleus, cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), Nucleic acid-binding, OB-fold (InterPro:IPR012340), ATPase, AAA+ type, core (InterPro:IPR003593), DNA-dependent ATPase MCM (InterPro:IPR001208), DNA-dependent ATPase MCM, conserved site (InterPro:IPR018525), MCM protein 7 (InterPro:IPR008050); BEST Arabidopsis thaliana protein match is: minichromosome maintenance (MCM2/3/5) family protein (TAIR:AT5G44635.1); Has 4609 Blast hits to 4442 proteins in 796 species: Archae - 407; Bacteria - 660; Metazoa - 1239; Fungi - 945; Plants - 433; Viruses - 2; Other Eukaryotes - 923 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT4G02060.2

Model type: Protein coding
Short Description: Minichromosome maintenance (MCM2/3/5) family protein
Curator Summary: Member of the minichromosome maintenance complex, involved in DNA replication initiation. Abundant in proliferating and endocycling tissues. Localized in the nucleus during G1, S and G2 phases of the cell cycle, and are released into the cytoplasmic compartment during mitosis. Binds chromatin.
Computational Description: PROLIFERA (PRL); CONTAINS InterPro DOMAIN/s: Nucleic acid-binding, OB-fold-like (InterPro:IPR016027), Nucleic acid-binding, OB-fold (InterPro:IPR012340), ATPase, AAA+ type, core (InterPro:IPR003593), DNA-dependent ATPase MCM (InterPro:IPR001208), DNA-dependent ATPase MCM, conserved site (InterPro:IPR018525), MCM protein 7 (InterPro:IPR008050); BEST Arabidopsis thaliana protein match is: minichromosome maintenance (MCM2/3/5) family protein (TAIR:AT5G44635.1).
Link: InterPro Scan - TAIR