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Mutant Lines
11-4729-1

11-4729-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

This line has NOT been observed.

Determination of insertion point(s)

5 prime edge [+] show sequence
1. Chromosome: 3; Position: 23452557 [Map]; Strand: w
  - AT3G63510.1 - [+] show detail - FMN-linked oxidoreductases superfamily protein
    Distance: 0; Region: exon12 (CDS)
  - AT3G63510.2 - [+] show detail - FMN-linked oxidoreductases superfamily protein
    Distance: 0; Region: exon12 (CDS)
  - AT3G63520.1 - [+] show detail - carotenoid cleavage dioxygenase 1
    Distance: -354; Region: promoter
Flanking Genome Sequence: 11-4729-1 - 5-prime edge
```
GTGTTGAGTG GTTGAATTAT GTATTGCAGA GTGTTGGATC GTTGCTGGAA 
GAAAGCT
```

3 prime edge [+] show sequence

Chromosome: 3; Position: 23452441 [Map]; Strand: w
- AT3G63510.1 - [+] show detail - FMN-linked oxidoreductases superfamily protein
  Distance: 0; Region: exon11 (CDS)
- AT3G63510.2 - [+] show detail - FMN-linked oxidoreductases superfamily protein
  Distance: 0; Region: exon11 (CDS)
- AT3G63520.1 - [+] show detail - carotenoid cleavage dioxygenase 1
  Distance: -470; Region: promoter

CTCAACACCA GTTACAAACT TTCTAACGTG TTACCACCAA TATATCACAC 
ACCCTGCACT CCTTGAAAGC TGCGTCTGCT CTCCGCTTCC ACAAGCTATT 
TCCATTTTCT GAATGGAAAA GGTTTAGCAG AGGCTGCAAT CAATTGAAAC 
CATCTTCAGT TCACACACAA GACTTGTATT ACTTTTCTCA ACACCTACAG 
CTTTCTTGTT NTAAACCACA CCTACCTTCA CCAAATCCCT CACATTAGGC 
CTCCCGTTTC CATGTGTTCC TAACACAGAA TCTCCATATA CTTGATATTG 
CTCAAGAACC TGCCCAACAA CACGCAGATT TTAGTTAGCT TATTGATAAG 
TTTGACAGAC AAAGCATCTC AAAAGCTGAA TATTTCCACG GGAACCTGTC 
CGGCGTGTAA AGGCCACTGC TTGGTACACC ATAAAACTTG NAGGNGGTNN 
AACATGGTNC NAAANCNTTT TGGCCNTGGG GCTNGNCAAA GGANAAAANA 
NNGAANTNAA AAAAAAANGG GGNTTTTGAN TANAAACCCA AAAAAAATTN 
GNNCCAAATT TTCCCNANTC TTNGGGGGNT NGGNNCNNNG GCCCNCNNCA 
NANATCCAGG NNGCNCCTNC CTTTNNGGGN GGGGGTTANA CTTTNAAAAN 
NNAAAAAAAN TTTTNGNGGC TTNNCCCCGG GGGGGGNNAA AAAAAAAACT 
NTNTTTTTNT TNTCNCNAAA A

AT3G63510.1

Model type: Protein coding
Short Description: FMN-linked oxidoreductases superfamily protein
Computational Description: FMN-linked oxidoreductases superfamily protein; FUNCTIONS IN: tRNA dihydrouridine synthase activity, FAD binding, catalytic activity; INVOLVED IN: regulation of nitrogen utilization, tRNA processing, oxidation reduction, metabolic process; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aldolase-type TIM barrel (InterPro:IPR013785), tRNA-dihydrouridine synthase (InterPro:IPR001269); BEST Arabidopsis thaliana protein match is: Aldolase-type TIM barrel family protein (TAIR:AT5G47970.2); Has 11920 Blast hits to 11916 proteins in 2583 species: Archae - 58; Bacteria - 8071; Metazoa - 278; Fungi - 218; Plants - 160; Viruses - 0; Other Eukaryotes - 3135 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G63510.2

Model type: Protein coding
Short Description: FMN-linked oxidoreductases superfamily protein
Computational Description: FMN-linked oxidoreductases superfamily protein; FUNCTIONS IN: tRNA dihydrouridine synthase activity, FAD binding, catalytic activity; INVOLVED IN: regulation of nitrogen utilization, tRNA processing, oxidation reduction, metabolic process; EXPRESSED IN: 18 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Aldolase-type TIM barrel (InterPro:IPR013785), tRNA-dihydrouridine synthase (InterPro:IPR001269); BEST Arabidopsis thaliana protein match is: Aldolase-type TIM barrel family protein (TAIR:AT5G47970.2); Has 11664 Blast hits to 11660 proteins in 2553 species: Archae - 58; Bacteria - 8007; Metazoa - 234; Fungi - 152; Plants - 146; Viruses - 0; Other Eukaryotes - 3067 (source: NCBI BLink).
Link: InterPro Scan - TAIR

AT3G63520.1

Model type: Protein coding
Short Description: carotenoid cleavage dioxygenase 1
Curator Summary: Encodes a protein with 9-cis-epoxycarotenoid dioxygenase activity. The enzyme was shown to act on a variety of carotenoid including β-carotene, lutein, zeaxanthin, and all-trans-violaxanthin. When those compounds are used as substrates, the major reaction product detected is a C14 dialdehyde: 4,9-dimethyldodeca-2,4,6,8,10-pentaene-1,12-dial. The enzyme did not cleave as efficiently carotenoids containing 9-cis-double or allenic bonds.
Computational Description: carotenoid cleavage dioxygenase 1 (CCD1); FUNCTIONS IN: 9-cis-epoxycarotenoid dioxygenase activity; INVOLVED IN: response to water deprivation, carotene catabolic process, xanthophyll catabolic process, carotenoid catabolic process; LOCATED IN: plasma membrane, vacuole, cytoplasm; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Carotenoid oxygenase (InterPro:IPR004294); BEST Arabidopsis thaliana protein match is: nine-cis-epoxycarotenoid dioxygenase 4 (TAIR:AT4G19170.1); Has 2926 Blast hits to 2896 proteins in 500 species: Archae - 16; Bacteria - 808; Metazoa - 317; Fungi - 194; Plants - 866; Viruses - 0; Other Eukaryotes - 725 (source: NCBI BLink).
Link: InterPro Scan - TAIR