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11-2474-1

RIKEN Arabidopsis Ds transposon mutant lines

Phenotypes

Morphological phenotypes

Specific phenotypes were NOT observed.

Determination of insertion point(s)

  • 5 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3486553 [Map]; Strand: c
      • AT1G10560.1 - [+] show detail - plant U-box 18
        Distance: 0; Region: exon1 (CDS)
    Flanking Genome Sequence: 11-2474-1 - 5-prime edge 
    CTGATNAGCG CACNANCCTT GNTGCTTCCT CNATATTCGC CGNTACTTAA 
    CACCGTGTNA AGCAGACCCC ANGACCAACG GAATTAGACA GCCCGGGGNG 
    NAA

  • 3 prime edge [+] show sequence

    1. Chromosome: 1; Position: 3486669 [Map]; Strand: c
      • AT1G10560.1 - [+] show detail - plant U-box 18
        Distance: 0; Region: exon1 (CDS)
      • AT1G10570.2 - [+] show detail - Cysteine proteinases superfamily protein
        Distance: -915; Region: promoter
      • AT1G10570.1 - [+] show detail - Cysteine proteinases superfamily protein
        Distance: -925; Region: promoter
    Flanking Genome Sequence: 11-2474-1 - 3-prime edge 
    CTGATCAGAA TGATCCATGA GTTCCAGGAG AGGAAAACCG GTTCGGTTGA 
    ACCGAATCTT CAAAGAGGAC GGTTCGTTCA CGCCTGGTGA TTTTGGTACA 
    CAGTAACTCT TGAGTATTTT AAAGAGTTAT TTACCATAAA GAAAACTAAA 
    TAATTTTGGT TAAGGGTGAA ATTCTTTTAT TAATTTTTAT GTAAAGGGTT 
    TAGTGTTTGT ATATTTAGCT CGGGTTTTAT TTTCATAAAC CAAAGAGCTA 
    CTGGGATGGC AATAAGCT

AT1G10560.1

Model type
Protein coding
Short Description
plant U-box 18
Curator Summary
Encodes a protein containing a UND, a U-box, and an ARM domain. This protein has E3 ubiquitin ligase activity based on in vitro assays.
Computational Description
plant U-box 18 (PUB18); FUNCTIONS IN: ubiquitin-protein ligase activity; INVOLVED IN: protein ubiquitination; LOCATED IN: ubiquitin ligase complex; EXPRESSED IN: 9 plant structures; EXPRESSED DURING: 4 anthesis, LP.10 ten leaves visible, petal differentiation and expansion stage; CONTAINS InterPro DOMAIN/s: Zinc finger, RING-type (InterPro:IPR001841), U box domain (InterPro:IPR003613), Armadillo-like helical (InterPro:IPR011989), Armadillo (InterPro:IPR000225), Armadillo-type fold (InterPro:IPR016024); BEST Arabidopsis thaliana protein match is: ARM repeat superfamily protein (TAIR:AT1G60190.1); Has 3089 Blast hits to 2773 proteins in 228 species: Archae - 0; Bacteria - 22; Metazoa - 252; Fungi - 338; Plants - 2172; Viruses - 3; Other Eukaryotes - 302 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G10570.1

Model type
Protein coding
Short Description
Cysteine proteinases superfamily protein
Curator Summary
Encodes a deSUMOylating enzyme. In vitro it has both peptidase activity and isopeptidase activity: it can cleave the C-terminal residues from SUMO to activate it for attachment to a target protein and it can also act on the isopeptide bond between SUMO and another protein. sYFP:OTS2 protein accumulates in nuclei in a punctate pattern. Double mutant analysis with ULP1D/OTS1 indicates that these genes are involved in salt stress responses and flowering time regulation.
Computational Description
OVERLY TOLERANT TO SALT 2 (OTS2); CONTAINS InterPro DOMAIN/s: Peptidase C48, SUMO/Sentrin/Ubl1 (InterPro:IPR003653); BEST Arabidopsis thaliana protein match is: UB-like protease 1D (TAIR:AT1G60220.1); Has 1265 Blast hits to 1250 proteins in 204 species: Archae - 0; Bacteria - 0; Metazoa - 616; Fungi - 251; Plants - 239; Viruses - 3; Other Eukaryotes - 156 (source: NCBI BLink).
Link
InterPro Scan - TAIR

AT1G10570.2

Model type
Protein coding
Short Description
Cysteine proteinases superfamily protein
Curator Summary
Encodes a deSUMOylating enzyme. In vitro it has both peptidase activity and isopeptidase activity: it can cleave the C-terminal residues from SUMO to activate it for attachment to a target protein and it can also act on the isopeptide bond between SUMO and another protein. sYFP:OTS2 protein accumulates in nuclei in a punctate pattern. Double mutant analysis with ULP1D/OTS1 indicates that these genes are involved in salt stress responses and flowering time regulation.
Computational Description
OVERLY TOLERANT TO SALT 2 (OTS2); CONTAINS InterPro DOMAIN/s: Peptidase C48, SUMO/Sentrin/Ubl1 (InterPro:IPR003653); BEST Arabidopsis thaliana protein match is: UB-like protease 1D (TAIR:AT1G60220.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
Link
InterPro Scan - TAIR